TY - JOUR
T1 - Airborne particulate matter PM
AU - Sierra-Vargas, Martha Patricia
AU - Guzman-Grenfell, Alberto Martin
AU - Blanco-Jimenez, Salvador
AU - Sepulveda-Sanchez, Jose David
AU - Bernabe-Cabanillas, Rosa Maria
AU - Cardenas-Gonzalez, Beatriz
AU - Ceballos, Guillermo
AU - Hicks, Juan Jose
N1 - Funding Information:
We thank Ms. Maria del Carmen Figueroa of Departamento de Investi-gación en Tabaquismo for performing the spirometry and also the field/laboratory technicians who worked on this project. We owe a great deal to our study subjects. This work was supported by CONACYT-SEMARNAT grant FOSEMARNAT-2004-01-27. The research described in this article was conducted according to the principles of the Declaration of Helsinki.
PY - 2009
Y1 - 2009
N2 - Background. The Mexico City Metropolitan Area is densely populated, and toxic air pollutants are generated and concentrated at a higher rate because of its geographic characteristics. It is well known that exposure to particulate matter, especially to fine and ultra-fine particles, enhances the risk of cardio-respiratory diseases, especially in populations susceptible to oxidative stress. The aim of this study was to evaluate the effect of fine particles on the respiratory burst of circulating neutrophils from asthmatic patients living in Mexico City. Methods. In total, 6 subjects diagnosed with mild asthma and 11 healthy volunteers were asked to participate. Neutrophils were isolated from peripheral venous blood and incubated with fine particles, and the generation of reactive oxygen species was recorded by chemiluminescence. We also measured plasma lipoperoxidation susceptibility and plasma myeloperoxidase and paraoxonase activities by spectrophotometry. Results. Asthmatic patients showed significantly lower plasma paraoxonase activity, higher susceptibility to plasma lipoperoxidation and an increase in myeloperoxidase activity that differed significantly from the control group. In the presence of fine particles, neutrophils from asthmatic patients showed an increased tendency to generate reactive oxygen species after stimulation with fine particles (PM2.5). Conclusion. These findings suggest that asthmatic patients have higher oxidation of plasmatic lipids due to reduced antioxidant defense. Furthermore, fine particles tended to increase the respiratory burst of blood human neutrophils from the asthmatic group. On the whole, increased myeloperoxidase activity and susceptibility to lipoperoxidation with a concomitant decrease in paraoxonase activity in asthmatic patients could favor lung infection and hence disrupt the control of asthmatic crises.
AB - Background. The Mexico City Metropolitan Area is densely populated, and toxic air pollutants are generated and concentrated at a higher rate because of its geographic characteristics. It is well known that exposure to particulate matter, especially to fine and ultra-fine particles, enhances the risk of cardio-respiratory diseases, especially in populations susceptible to oxidative stress. The aim of this study was to evaluate the effect of fine particles on the respiratory burst of circulating neutrophils from asthmatic patients living in Mexico City. Methods. In total, 6 subjects diagnosed with mild asthma and 11 healthy volunteers were asked to participate. Neutrophils were isolated from peripheral venous blood and incubated with fine particles, and the generation of reactive oxygen species was recorded by chemiluminescence. We also measured plasma lipoperoxidation susceptibility and plasma myeloperoxidase and paraoxonase activities by spectrophotometry. Results. Asthmatic patients showed significantly lower plasma paraoxonase activity, higher susceptibility to plasma lipoperoxidation and an increase in myeloperoxidase activity that differed significantly from the control group. In the presence of fine particles, neutrophils from asthmatic patients showed an increased tendency to generate reactive oxygen species after stimulation with fine particles (PM2.5). Conclusion. These findings suggest that asthmatic patients have higher oxidation of plasmatic lipids due to reduced antioxidant defense. Furthermore, fine particles tended to increase the respiratory burst of blood human neutrophils from the asthmatic group. On the whole, increased myeloperoxidase activity and susceptibility to lipoperoxidation with a concomitant decrease in paraoxonase activity in asthmatic patients could favor lung infection and hence disrupt the control of asthmatic crises.
UR - http://www.scopus.com/inward/record.url?scp=68249158552&partnerID=8YFLogxK
U2 - 10.1186/1745-6673-4-17
DO - 10.1186/1745-6673-4-17
M3 - Artículo
C2 - 19563660
SN - 1745-6673
VL - 4
JO - Journal of Occupational Medicine and Toxicology
JF - Journal of Occupational Medicine and Toxicology
IS - 1
M1 - 17
ER -