TY - JOUR
T1 - Adult, but not neonatal, human lymphoid progenitors respond to TLR9 ligation by producing functional NK-like cells
AU - Vadillo, Eduardo
AU - Dorantes-Acosta, Elisa
AU - Arriaga-Pizano, Lourdes
AU - Chavez-Gonzalez, Antonieta
AU - Reyes-Maldonado, Elba
AU - Garrett, Karla P.
AU - Mayani, Héctor
AU - Kincade, Paul W.
AU - Pelayo, Rosana
N1 - Funding Information:
This work was supported by the Consejo Nacional de Ciencia y Tecnología , México (Grant No. CB-2006-C01-61274 ) and the Mexican Institute for Social Security , IMSS (Grant Nos. R-2006-3602-16 and 2008-785-044 ). E. Vadillo received support from Consejo Nacional de Ciencia y Tecnología for M.Sc. studies (Scholarship No. B081650).
PY - 2014/7
Y1 - 2014/7
N2 - Remarkable progress has been made in characterizing factors controlling lineage fate decisions of primitive progenitors that initiate the lymphoid program in bone marrow. However, the understanding of neonatal/adult differences in environmental signals that influence differentiation pathway stability is still incomplete. Our recent findings suggest that Toll-like receptors provide a mechanism for producing cells of the innate immune system from early stages of lymphoid development in mice. We now show that both human early multilymphoid progenitors and more differentiated lymphoid progenitors from normal adult bone marrow express TLR9. Furthermore, they respond to its ligation by upregulating the expression of IL-15Rβ (CD122) and accelerating the production of functional natural killer (NK)-like cells. Proliferation of the presumed equivalent progenitor cells from umbilical cord blood was stimulated by CpG-containing oligonucleotides or herpes simplex virus, but the already robust NK-cell formation was unchanged. This new information adds to other known differences between neonatal and adult lymphoid progenitors and suggests only the latter replenish innate NK-like cells in response to Toll-like receptor agonists.
AB - Remarkable progress has been made in characterizing factors controlling lineage fate decisions of primitive progenitors that initiate the lymphoid program in bone marrow. However, the understanding of neonatal/adult differences in environmental signals that influence differentiation pathway stability is still incomplete. Our recent findings suggest that Toll-like receptors provide a mechanism for producing cells of the innate immune system from early stages of lymphoid development in mice. We now show that both human early multilymphoid progenitors and more differentiated lymphoid progenitors from normal adult bone marrow express TLR9. Furthermore, they respond to its ligation by upregulating the expression of IL-15Rβ (CD122) and accelerating the production of functional natural killer (NK)-like cells. Proliferation of the presumed equivalent progenitor cells from umbilical cord blood was stimulated by CpG-containing oligonucleotides or herpes simplex virus, but the already robust NK-cell formation was unchanged. This new information adds to other known differences between neonatal and adult lymphoid progenitors and suggests only the latter replenish innate NK-like cells in response to Toll-like receptor agonists.
UR - http://www.scopus.com/inward/record.url?scp=84904887218&partnerID=8YFLogxK
U2 - 10.1016/j.exphem.2014.03.008
DO - 10.1016/j.exphem.2014.03.008
M3 - Artículo
C2 - 24721609
SN - 0301-472X
VL - 42
SP - 562-573.e3
JO - Experimental Hematology
JF - Experimental Hematology
IS - 7
ER -