TY - JOUR
T1 - Acute intravenous injection and short-term oral administration of NG-nitro-l-arginine methyl ester to the rat provoke increased pressor responses to agonists and hypertension, but not inhibition of acetylcholine-induced hypotensive responses
AU - López, Ruth M.
AU - Pérez, Teresa
AU - Castillo, Carlos
AU - Castillo, María C.
AU - Castillo, Enrique F.
PY - 2011/6
Y1 - 2011/6
N2 - In experiments in vivo, we studied whether the endothelial dysfunction induced by nitric oxide (NO) synthesis inhibition is simultaneously or sequentially manifested as a reduced level of endothelium-dependent agonist-induced vasodilatation, an increased responsiveness to vasoconstrictors, and hypertension. Vascular responses to acetylcholine, phenylephrine, and angiotensin II were measured in pithed rats after acute intravenous injection (100mg/kg) and short-term oral administration of NG-nitro-l-arginine methyl ester (l-NAME; 60mg/kg per day) for 1 and 3days (l-NAME1d and l-NAME3d, respectively). Pithed rats were chosen because drug-induced cardiovascular responses reflect only peripheral effects. Parallel experiments examined mean arterial pressure (MAP) values in anesthetized rats. After short-term l-NAME1d and l-NAME3d treatments, the MAP was significantly elevated in anesthetized but not pithed rats. Acute intravenous administration of l-NAME elevated MAP in pithed rats. Intravenous infusion of phenylephrine was used to compensate for the pressor response induced by l-NAME in pithed animals. The maximum decrease and duration of the hypotensive responses to acetylcholine were unaltered by the acute and both short-term l-NAME treatments in pithed rats. These treatments, on the other hand, increased phenylephrine- and angiotensin II-induced pressor responses in pithed animals. In isolated aortic rings prepared from pithed rats treated acutely and short-term with l-NAME, acetylcholine-induced relaxations were inhibited. Thus, the inhibition of NO-dependent vasodilator tone after acute intravenous injection and short-term oral l-NAME administration may be associated with vascular smooth muscle hyper-responsiveness to pressor agonists and hypertension, whereas the hypotensive responses to acetylcholine could not be associated with the l-NAME-induced endothelial dysfunction in pithed rats.
AB - In experiments in vivo, we studied whether the endothelial dysfunction induced by nitric oxide (NO) synthesis inhibition is simultaneously or sequentially manifested as a reduced level of endothelium-dependent agonist-induced vasodilatation, an increased responsiveness to vasoconstrictors, and hypertension. Vascular responses to acetylcholine, phenylephrine, and angiotensin II were measured in pithed rats after acute intravenous injection (100mg/kg) and short-term oral administration of NG-nitro-l-arginine methyl ester (l-NAME; 60mg/kg per day) for 1 and 3days (l-NAME1d and l-NAME3d, respectively). Pithed rats were chosen because drug-induced cardiovascular responses reflect only peripheral effects. Parallel experiments examined mean arterial pressure (MAP) values in anesthetized rats. After short-term l-NAME1d and l-NAME3d treatments, the MAP was significantly elevated in anesthetized but not pithed rats. Acute intravenous administration of l-NAME elevated MAP in pithed rats. Intravenous infusion of phenylephrine was used to compensate for the pressor response induced by l-NAME in pithed animals. The maximum decrease and duration of the hypotensive responses to acetylcholine were unaltered by the acute and both short-term l-NAME treatments in pithed rats. These treatments, on the other hand, increased phenylephrine- and angiotensin II-induced pressor responses in pithed animals. In isolated aortic rings prepared from pithed rats treated acutely and short-term with l-NAME, acetylcholine-induced relaxations were inhibited. Thus, the inhibition of NO-dependent vasodilator tone after acute intravenous injection and short-term oral l-NAME administration may be associated with vascular smooth muscle hyper-responsiveness to pressor agonists and hypertension, whereas the hypotensive responses to acetylcholine could not be associated with the l-NAME-induced endothelial dysfunction in pithed rats.
KW - Endothelium dysfunction
KW - Nitric oxide
KW - Pithed rat
UR - http://www.scopus.com/inward/record.url?scp=79954625155&partnerID=8YFLogxK
U2 - 10.1111/j.1472-8206.2010.00852.x
DO - 10.1111/j.1472-8206.2010.00852.x
M3 - Artículo
SN - 0767-3981
VL - 25
SP - 333
EP - 342
JO - Fundamental and Clinical Pharmacology
JF - Fundamental and Clinical Pharmacology
IS - 3
ER -