TY - JOUR
T1 - Activity of quinolizidine alkaloids from three Mexican Lupinus against the lepidopteran crop pest Spodoptera frugiperda
AU - Bermúdez-Torres, Kalina
AU - Martínez Herrera, Jorge
AU - Figueroa Brito, Rodolfo
AU - Wink, Michael
AU - Legal, Luc
N1 - Funding Information:
Acknowledgements Peter Winterton is thanked for his help in correcting English. This study was supported by CONACYT (26411-N) and CGPI (978038). Laura Lina is greatly thanked for providing Spodoptera frugiperda eggs.
PY - 2009/6
Y1 - 2009/6
N2 - Bitter lupins (Lupinus spp.) are not used as a protein source because of their toxicity. However, they may have alternative uses as potential sources of natural insecticides. Quinolizidine alkaloids (QA) of three Mexican Lupinus species (Fabaceae): L. montanus (HBK), L. stipulatus (Agardh) and L. aschenbornii (Schauer), were analyzed by capillary Gas Chromatography-Mass Spectrometry. Sparteine was found in high amounts in both L. montanus and L. aschenbornii while the major alkaloids in L. stipulatus extract were aphylline and an epiaphylline-like compound. Alkaloid extracts were tested for their insecticidal activity using larvae of the Fall Armyworm, Spodoptera frugiperda (Smith); (Lepidoptera, Noctuidae) as a model pest. We compared LD50 values and mean weight of caterpillars fed with alkaloid extracts of the three species studied with those of sparteine, a widespread QA found in various lupin species. Extracts of L. montanus and L. aschenbornii were found to be as effective as sparteine and extracts L. stipulatus were found to be the most toxic against the larvae of S. frugiperda. This suggests that the various QA act differently on caterpillars, and could be used to control Spodoptera populations.
AB - Bitter lupins (Lupinus spp.) are not used as a protein source because of their toxicity. However, they may have alternative uses as potential sources of natural insecticides. Quinolizidine alkaloids (QA) of three Mexican Lupinus species (Fabaceae): L. montanus (HBK), L. stipulatus (Agardh) and L. aschenbornii (Schauer), were analyzed by capillary Gas Chromatography-Mass Spectrometry. Sparteine was found in high amounts in both L. montanus and L. aschenbornii while the major alkaloids in L. stipulatus extract were aphylline and an epiaphylline-like compound. Alkaloid extracts were tested for their insecticidal activity using larvae of the Fall Armyworm, Spodoptera frugiperda (Smith); (Lepidoptera, Noctuidae) as a model pest. We compared LD50 values and mean weight of caterpillars fed with alkaloid extracts of the three species studied with those of sparteine, a widespread QA found in various lupin species. Extracts of L. montanus and L. aschenbornii were found to be as effective as sparteine and extracts L. stipulatus were found to be the most toxic against the larvae of S. frugiperda. This suggests that the various QA act differently on caterpillars, and could be used to control Spodoptera populations.
KW - Botanical insecticides
KW - Insecticidal activity
KW - Lupinus aschenbornii
KW - Lupinus montanus
KW - Lupinus stipulatus
KW - Sparteine
UR - http://www.scopus.com/inward/record.url?scp=67349184332&partnerID=8YFLogxK
U2 - 10.1007/s10526-008-9180-y
DO - 10.1007/s10526-008-9180-y
M3 - Artículo
SN - 1386-6141
VL - 54
SP - 459
EP - 466
JO - BioControl
JF - BioControl
IS - 3
ER -