TY - JOUR
T1 - Acid increases inflammatory pain in rats
T2 - Effect of local peripheral ASICs inhibitors
AU - Rocha-González, Héctor I.
AU - Herrejon-Abreu, Emma B.
AU - López-Santillán, Francisco J.
AU - García-López, Blanca E.
AU - Murbartián, Janet
AU - Granados-Soto, Vinicio
N1 - Funding Information:
This work was done at the Departamento de Farmacobiología, Centro de Investigación y de Estudios Avanzados, Sede Sur. The authors greatly appreciate the technical and bibliographic assistance of Guadalupe C. Vidal-Cantú and Héctor Vázquez, respectively. Héctor I. Rocha-González is a Conacyt fellow. Francisco J. López-Santillán and Blanca E. García-López are summer visiting students from Facultad de Química, UNAM and Facultad de Ciencias Químicas, Universidad Autónoma de Sinaloa, respectively. This work is part of the B.Sc. dissertation of Emma B. Herrejón-Abreu. Partially supported by Conacyt, grant 59879 (VGS).
PY - 2009/1/28
Y1 - 2009/1/28
N2 - The purpose of this study was to assess the possible antinociceptive effect of the acid sensing ion channels (ASICs) inhibitors amiloride and benzamil after local peripheral administration in three models of inflammatory pain in rats. Reduction of pH, from 7.4 to 5.8 units, significantly increased the flinching/licking behavior induced by either 0.1% serotonin, 0.1% capsaicin or 0.5% formalin. Local peripheral ipsilateral, but not contralateral, injection of amiloride or benzamil significantly reduced nociceptive behaviors (flinching and licking/lifting) induced by serotonin, capsaicin or formalin in acidic conditions (pH 6.2). Interestingly, benzamil produced antinociception at low doses (0.001-0.1 μM/paw) while higher doses (1 μM/paw) did not affect capsaicin- or formalin-induced licking/lifting. Our data suggest that local peripheral inhibition of ASICs play an important role in inflammatory pain.
AB - The purpose of this study was to assess the possible antinociceptive effect of the acid sensing ion channels (ASICs) inhibitors amiloride and benzamil after local peripheral administration in three models of inflammatory pain in rats. Reduction of pH, from 7.4 to 5.8 units, significantly increased the flinching/licking behavior induced by either 0.1% serotonin, 0.1% capsaicin or 0.5% formalin. Local peripheral ipsilateral, but not contralateral, injection of amiloride or benzamil significantly reduced nociceptive behaviors (flinching and licking/lifting) induced by serotonin, capsaicin or formalin in acidic conditions (pH 6.2). Interestingly, benzamil produced antinociception at low doses (0.001-0.1 μM/paw) while higher doses (1 μM/paw) did not affect capsaicin- or formalin-induced licking/lifting. Our data suggest that local peripheral inhibition of ASICs play an important role in inflammatory pain.
KW - ASICs [acid sensing ion channels]
KW - Amiloride
KW - Benzamil
KW - Inflammatory pain
KW - Peripheral sensitization
UR - http://www.scopus.com/inward/record.url?scp=58249118002&partnerID=8YFLogxK
U2 - 10.1016/j.ejphar.2008.12.017
DO - 10.1016/j.ejphar.2008.12.017
M3 - Artículo
C2 - 19109946
SN - 0014-2999
VL - 603
SP - 56
EP - 61
JO - European Journal of Pharmacology
JF - European Journal of Pharmacology
IS - 1-3
ER -