TY - JOUR
T1 - A two-reservoir, hollow-fiber bioreactor for the study of mixed-population dynamics
T2 - Design aspects and validation of the approach
AU - Manjarrez, Edgar Salgado
AU - Albasi, Claire
AU - Riba, Jean Pierre
PY - 2000/8/20
Y1 - 2000/8/20
N2 - A two-reservoir, membrane bioreactor for carrying out studies of mixed-population dynamics in batch fermentations is presented. Mixing requirements and design aspects for the validity of the approach are given and discussed. Equations describing mixing times between the reservoirs are presented and compared to the experimental results. The validity of the approach is demonstrated by the study of an amensalistic-type interaction, the protein-mediated killer phenomenon between two Saccharomyces cerevisiae strains. The validation consisted in the comparison between the results obtained in actual mixed culture and the results obtained by keeping the strains separated. A good agreement was found which demonstrates the viability of the designed bioreactor.
AB - A two-reservoir, membrane bioreactor for carrying out studies of mixed-population dynamics in batch fermentations is presented. Mixing requirements and design aspects for the validity of the approach are given and discussed. Equations describing mixing times between the reservoirs are presented and compared to the experimental results. The validity of the approach is demonstrated by the study of an amensalistic-type interaction, the protein-mediated killer phenomenon between two Saccharomyces cerevisiae strains. The validation consisted in the comparison between the results obtained in actual mixed culture and the results obtained by keeping the strains separated. A good agreement was found which demonstrates the viability of the designed bioreactor.
KW - Killer phenomenon
KW - Membrane reactor
KW - Mixed culture dynamics
UR - http://www.scopus.com/inward/record.url?scp=0034238094&partnerID=8YFLogxK
U2 - 10.1002/1097-0290(20000820)69:4<401::AID-BIT6>3.0.CO;2-3
DO - 10.1002/1097-0290(20000820)69:4<401::AID-BIT6>3.0.CO;2-3
M3 - Artículo
C2 - 10862678
AN - SCOPUS:0034238094
SN - 0006-3592
VL - 69
SP - 401
EP - 408
JO - Biotechnology and Bioengineering
JF - Biotechnology and Bioengineering
IS - 4
ER -