TY - CHAP
T1 - A Three-Dimensional Culture-Based Assay to Detect Early Stages of Vasculogenic Mimicry in Ovarian Cancer Cells
AU - Salinas-Vera, Yarely M.
AU - Gallardo-Rincón, Dolores
AU - Ruíz-García, Erika
AU - Marchat, Laurence A.
AU - Valdés, Jesús
AU - Vázquez-Calzada, Carlos
AU - López-Camarillo, César
N1 - Publisher Copyright:
© 2022, Springer Science+Business Media, LLC, part of Springer Nature.
PY - 2022
Y1 - 2022
N2 - Vasculogenic mimicry is a cellular mechanism in which tumor cells grow and align forming complex three-dimensional (3D) channel-like structures in a hypoxic microenvironment. This phenomenon represents a novel oxygen, nutrient, and blood supply, in a similar way as occurs in classic angiogenesis. Vasculogenic mimicry has been described in numerous clinical tumors including breast, prostate, lung, and ovarian cancers where it is associated with poor prognosis; thus, it is considered as a hallmark of highly aggressive and metastatic tumors. Here, we describe a simple method to model the in vitro formation of three-dimensional cellular networks over Matrigel in SKOV3 ovarian cancer cells representing the early stages of vasculogenic mimicry.
AB - Vasculogenic mimicry is a cellular mechanism in which tumor cells grow and align forming complex three-dimensional (3D) channel-like structures in a hypoxic microenvironment. This phenomenon represents a novel oxygen, nutrient, and blood supply, in a similar way as occurs in classic angiogenesis. Vasculogenic mimicry has been described in numerous clinical tumors including breast, prostate, lung, and ovarian cancers where it is associated with poor prognosis; thus, it is considered as a hallmark of highly aggressive and metastatic tumors. Here, we describe a simple method to model the in vitro formation of three-dimensional cellular networks over Matrigel in SKOV3 ovarian cancer cells representing the early stages of vasculogenic mimicry.
KW - Engelbreth
KW - Holm
KW - Ovarian cancer
KW - Swarm extracellular matrix extract
KW - Three-dimensional culture
KW - Vasculogenic mimicry
KW - miR-765
KW - microRNAs
UR - http://www.scopus.com/inward/record.url?scp=85133499188&partnerID=8YFLogxK
U2 - 10.1007/978-1-0716-2403-6_6
DO - 10.1007/978-1-0716-2403-6_6
M3 - Capítulo
C2 - 35771418
AN - SCOPUS:85133499188
T3 - Methods in Molecular Biology
SP - 53
EP - 60
BT - Methods in Molecular Biology
PB - Humana Press Inc.
ER -
