TY - JOUR
T1 - A new set of microsatellite loci for Leptonycteris yerbabuenae and cross species amplification with other glossophagines
AU - Romero-Meza, Jose Antonio
AU - Lance, Stacey L.
AU - Ortega, Jorge
N1 - Funding Information:
Acknowledgments Financial support was provided by FOMIX-Campeche (CONACyT) 95900. Tissue samples were obtained from L. León (Museo de Zoología, Facultad de Ciencias, UNAM), A. Rendón (Instituto Tecnológico de la Cuenca del Papaloapan), C. López (CIIDIR-Durango, IPN), and O. Gaona/R. S. Galicia (Instituto de Ecología, UNAM). We thank the field assistance provided by I. Campos and A. Hernández-Dávila. Manuscript preparation was partially supported by the DOE under Award Number DE-FC09-07SR22506 to the University of Georgia Research Foundation.
PY - 2012/6
Y1 - 2012/6
N2 - A technique based on 454 sequencing of an enriched library was used to construct genomic libraries highly enriched for microsatellite loci for Leptonycteris yerbabuenae. Twenty-four polymorphic microsatellites were developed and tested as markers in the target species. We probed ascertainment bias in 5 different glossophagines (L. nivalis, Glossophaga leachii, G. soricina, Anoura geoffroyi, and Choeronycteris mexicana), indicating their potential utility for suitable studies of population genetics and other related analyses. Levels of expected heterozygosity were medium-low for all markers (mean HE = 0.147, range 0.008-0.46).
AB - A technique based on 454 sequencing of an enriched library was used to construct genomic libraries highly enriched for microsatellite loci for Leptonycteris yerbabuenae. Twenty-four polymorphic microsatellites were developed and tested as markers in the target species. We probed ascertainment bias in 5 different glossophagines (L. nivalis, Glossophaga leachii, G. soricina, Anoura geoffroyi, and Choeronycteris mexicana), indicating their potential utility for suitable studies of population genetics and other related analyses. Levels of expected heterozygosity were medium-low for all markers (mean HE = 0.147, range 0.008-0.46).
KW - 454 Sequencing
KW - Ascertainment bias
KW - Glossophagines
KW - Leptonycteris yerbabuenae
KW - Microsatellites
UR - http://www.scopus.com/inward/record.url?scp=84892839043&partnerID=8YFLogxK
U2 - 10.1007/s12686-011-9527-z
DO - 10.1007/s12686-011-9527-z
M3 - Artículo
SN - 1877-7252
VL - 4
SP - 291
EP - 294
JO - Conservation Genetics Resources
JF - Conservation Genetics Resources
IS - 2
ER -