TY - JOUR
T1 - A microbial consortium isolated from a crude oil sample that uses asphaltenes as a carbon and energy source
AU - Pineda-Flores, Gabriel
AU - Boll-Argüello, Gisela
AU - Lira-Galeana, Carlos
AU - Mesta-Howard, Ana María
PY - 2004/6
Y1 - 2004/6
N2 - A microbial consortium capable of mineralizing asphaltenes was obtained from the Maya crude oil. The enrichment system was built with a glass column reactor containing mineral medium supplied with asphaltenes as energy and carbon source. The consortium growth was evaluated in Casoy agar during 40 weeks. The steady- state phase of the enriched bacterial community was observed after 10 weeks when the culture reach 105 to 106 CFU ml -1. The isolates belong to bacterial genus reported for degradation of other hydrocarbons and they were identified as Corynebacterium sp., Bacillus sp., Brevibacillus sp. and Staphylococcus sp. The bacterial consortium growth was evaluated by a viable counts during 14 days exposed to different aeration, temperature, salinity, and pH conditions. The ability of the consortium to mineralize asphaltenes was evaluated using the method of ISO 9439 in glass column reactors of 20 x 3.2 cm during 13 days. Temperatures of 55°C and salinity of 1.8% were growth limiting. The respiration of the microbial consortium using asphaltenes as a sole carbon source (800 μmoles CO 2 in 13 days) was significantly higher than those of the samples containing only the microbial consortium (200 μmoles CO2) or only asphaltenes (300 μmoles CO2). These results indicated the existence of asphaltenes-degradating microbes in the crude oil and confirmed that the consortium could mineralize asphaltenes in conditions of room temperature, salinity of 100 ppm, aeration of 11 min-1 and pH of 7.4.
AB - A microbial consortium capable of mineralizing asphaltenes was obtained from the Maya crude oil. The enrichment system was built with a glass column reactor containing mineral medium supplied with asphaltenes as energy and carbon source. The consortium growth was evaluated in Casoy agar during 40 weeks. The steady- state phase of the enriched bacterial community was observed after 10 weeks when the culture reach 105 to 106 CFU ml -1. The isolates belong to bacterial genus reported for degradation of other hydrocarbons and they were identified as Corynebacterium sp., Bacillus sp., Brevibacillus sp. and Staphylococcus sp. The bacterial consortium growth was evaluated by a viable counts during 14 days exposed to different aeration, temperature, salinity, and pH conditions. The ability of the consortium to mineralize asphaltenes was evaluated using the method of ISO 9439 in glass column reactors of 20 x 3.2 cm during 13 days. Temperatures of 55°C and salinity of 1.8% were growth limiting. The respiration of the microbial consortium using asphaltenes as a sole carbon source (800 μmoles CO 2 in 13 days) was significantly higher than those of the samples containing only the microbial consortium (200 μmoles CO2) or only asphaltenes (300 μmoles CO2). These results indicated the existence of asphaltenes-degradating microbes in the crude oil and confirmed that the consortium could mineralize asphaltenes in conditions of room temperature, salinity of 100 ppm, aeration of 11 min-1 and pH of 7.4.
KW - Asphaltenes
KW - Biodegradation
KW - Microbial consortium
KW - Mineralization
KW - Petroleum
UR - http://www.scopus.com/inward/record.url?scp=3242794344&partnerID=8YFLogxK
U2 - 10.1023/B:BIOD.0000026476.03744.bb
DO - 10.1023/B:BIOD.0000026476.03744.bb
M3 - Artículo
SN - 0923-9820
VL - 15
SP - 145
EP - 151
JO - Biodegradation
JF - Biodegradation
IS - 3
ER -