TY - JOUR
T1 - A comparison of Lewis X and Lewis Y expression in Helicobacter pylori obtained from children and adults
AU - Muñoz, L.
AU - González-Valencia, G.
AU - Pérez-Pérez, G. I.
AU - Giono-Cerezo, S.
AU - Muñoz, O.
AU - Torres, J.
N1 - Funding Information:
Financial support: Coordinación de Investigación Médica, Instituto Mexicano del Seguro Social, and Consejo Nacional de Ciencia y Tecnologia, Mexico (grant 28040).
PY - 2001/4/1
Y1 - 2001/4/1
N2 - There are no reports, to our knowledge, on the expression of Lewis (Le) antigens in Helicobacter pylori isolates from children. The aim of this study was to compare the expression of Le antigens by H. pylori isolates from children and from adults. Totals of 278 clones from 22 children with recurrent abdominal pain and 293 clones from 22 adults with (n = 10) or without (n = 12) duodenal ulcer were studied. Expression of Lexand Leyantigens was determined by ELISA, using monoclonal anti-Le antibodies. The Le phenotype of the patients was determined in gastric juice with a hemagglutination assay. Clones expressing Lexwere more common in children than in adults (55.4% vs. 33.4%, respectively; P<.001), and Leywas more common in adults than in children (81.6% vs. 66%, respectively; P<.01). A trend analysis showed a significant decline in frequency of clones expressing Lexwith age (P = .021). In this community, expression of Le antigens differs in H. pylori isolates obtained from children versus adults.
AB - There are no reports, to our knowledge, on the expression of Lewis (Le) antigens in Helicobacter pylori isolates from children. The aim of this study was to compare the expression of Le antigens by H. pylori isolates from children and from adults. Totals of 278 clones from 22 children with recurrent abdominal pain and 293 clones from 22 adults with (n = 10) or without (n = 12) duodenal ulcer were studied. Expression of Lexand Leyantigens was determined by ELISA, using monoclonal anti-Le antibodies. The Le phenotype of the patients was determined in gastric juice with a hemagglutination assay. Clones expressing Lexwere more common in children than in adults (55.4% vs. 33.4%, respectively; P<.001), and Leywas more common in adults than in children (81.6% vs. 66%, respectively; P<.01). A trend analysis showed a significant decline in frequency of clones expressing Lexwith age (P = .021). In this community, expression of Le antigens differs in H. pylori isolates obtained from children versus adults.
UR - http://www.scopus.com/inward/record.url?scp=0035313204&partnerID=8YFLogxK
U2 - 10.1086/319296
DO - 10.1086/319296
M3 - Artículo
C2 - 11237846
SN - 0022-1899
VL - 183
SP - 1147
EP - 1151
JO - Journal of Infectious Diseases
JF - Journal of Infectious Diseases
IS - 7
ER -