TY - JOUR
T1 - Whiteleg shrimp (Litopenaeus vannamei, Boone, 1931) isotrypsins
T2 - Their genotype and modulation
AU - Sainz, Juan Carlos
AU - García-Carreño, Fernando L.
AU - Córdova-Murueta, Julio H.
AU - Cruz-Hernández, Pedro
PY - 2005/12/6
Y1 - 2005/12/6
N2 - Based on information about isotrypsins found by substrate-SDS-PAGE in the whiteleg shrimp Litopenaeus vannamei and their segregation, an explanation for the presence of three-trypsin phenotype was sought. Isotrypsins A, B, and C depend on two loci: locus β, which is homozygous, yielding isoenzyme C, and locus α, which is heterozygous, yielding isoenzymes A and B. Segregation in locus α in offsprings from 20 families was analyzed. Results demonstrated that isotrypsins were segregated according to Mendelian rules, and hence, external stimuli did not affect the phenotype. To corroborate this, the phenotype was evaluated in relation to changes in trypsin activity during a digestion process. Trypsin phenotypes found in shrimp feces were also analyzed. Results showed that phenotypes were conserved by individuals. Therefore, changes in trypsin activity found in previous investigations must be related to changes in the concentration of the established isotrypsins.
AB - Based on information about isotrypsins found by substrate-SDS-PAGE in the whiteleg shrimp Litopenaeus vannamei and their segregation, an explanation for the presence of three-trypsin phenotype was sought. Isotrypsins A, B, and C depend on two loci: locus β, which is homozygous, yielding isoenzyme C, and locus α, which is heterozygous, yielding isoenzymes A and B. Segregation in locus α in offsprings from 20 families was analyzed. Results demonstrated that isotrypsins were segregated according to Mendelian rules, and hence, external stimuli did not affect the phenotype. To corroborate this, the phenotype was evaluated in relation to changes in trypsin activity during a digestion process. Trypsin phenotypes found in shrimp feces were also analyzed. Results showed that phenotypes were conserved by individuals. Therefore, changes in trypsin activity found in previous investigations must be related to changes in the concentration of the established isotrypsins.
KW - Digestive enzymes
KW - Enzyme regulation
KW - Litopenaeus vannamei
KW - Trypsin
KW - Trypsin polymorphism
KW - Whiteleg shrimp
UR - http://www.scopus.com/inward/record.url?scp=27744442052&partnerID=8YFLogxK
U2 - 10.1016/j.jembe.2005.05.021
DO - 10.1016/j.jembe.2005.05.021
M3 - Artículo
SN - 0022-0981
VL - 326
SP - 105
EP - 113
JO - Journal of Experimental Marine Biology and Ecology
JF - Journal of Experimental Marine Biology and Ecology
IS - 1
ER -