TY - JOUR
T1 - Volatolome of the Female Genitourinary Area
T2 - Toward the Metabolome of Cervical Cancer
AU - Rodríguez-Esquivel, Miriam
AU - Rosales, Juan
AU - Castro, Rafael
AU - Apresa-García, Teresa
AU - Garay, Ónix
AU - Romero-Morelos, Pablo
AU - Marrero-Rodríguez, Daniel
AU - Taniguchi-Ponciano, Keiko
AU - López-Romero, Ricardo
AU - Guerrero-Flores, Héctor
AU - Morales, Betsabé
AU - Mendoza-Rodríguez, Mónica
AU - Mosso-Lara, Dejanira
AU - Núñez-Nolasco, Itzalia
AU - Castro-Alba, Paola
AU - Meza-Toledo, Sergio E.
AU - Salcedo, Mauricio
N1 - Publisher Copyright:
© 2018 IMSS
PY - 2018/1
Y1 - 2018/1
N2 - Background and aims: Different Volatile Organic Compounds (VOCs) obtained from several human fluids (volatolome) has been reported as potential biomarkers for a great variety of diseases including cancer. At present, volatolomic profile data of the female genital area is scarce. Methods: To identify the VOCs related to the female genitourinary area of healthy and Cervical Cancer (CC)-affected women used a pad, as a non-invasive tool for sample gathering was necessary. Used pads were analyzed by Gas Chromatography–Mass Spectrometry. The data were subjected to Principal Component Analysis looking for a possible spectrum of VOCs that could help identify CC-affected patients. The diagnostic role of the VOCs was validated through Receiver Operating Characteristic (ROC) analysis. The area below the curve and the diagnostic sensitivity and specificity values were also evaluated. Results: The data showed great differences between female cancer and healthy patients groups; most of these VOCs belonging to the alkanes chemical classes. A group of VOCs were identified as common among CC patients, while others VOCs for healthy females. The ROC curve showed an optimal reach to diagnosis (89%), returning a 93% rate for sensitivity and specificity, indicating the VOCs identified in the samples could differentiate cancer patients from healthy females. Conclusions: In summary, we have detected and identified specific VOCs from healthy women that are not present in CC-affected females and VOCs specific of CC-affected women. We are strengthening our findings to aid in the detection of VOCs that are potential biomarkers for cervical tumors.
AB - Background and aims: Different Volatile Organic Compounds (VOCs) obtained from several human fluids (volatolome) has been reported as potential biomarkers for a great variety of diseases including cancer. At present, volatolomic profile data of the female genital area is scarce. Methods: To identify the VOCs related to the female genitourinary area of healthy and Cervical Cancer (CC)-affected women used a pad, as a non-invasive tool for sample gathering was necessary. Used pads were analyzed by Gas Chromatography–Mass Spectrometry. The data were subjected to Principal Component Analysis looking for a possible spectrum of VOCs that could help identify CC-affected patients. The diagnostic role of the VOCs was validated through Receiver Operating Characteristic (ROC) analysis. The area below the curve and the diagnostic sensitivity and specificity values were also evaluated. Results: The data showed great differences between female cancer and healthy patients groups; most of these VOCs belonging to the alkanes chemical classes. A group of VOCs were identified as common among CC patients, while others VOCs for healthy females. The ROC curve showed an optimal reach to diagnosis (89%), returning a 93% rate for sensitivity and specificity, indicating the VOCs identified in the samples could differentiate cancer patients from healthy females. Conclusions: In summary, we have detected and identified specific VOCs from healthy women that are not present in CC-affected females and VOCs specific of CC-affected women. We are strengthening our findings to aid in the detection of VOCs that are potential biomarkers for cervical tumors.
KW - Biomarkers
KW - Cervical cancer
KW - Genitourinary area
KW - Non-invasive
KW - Volatolome
UR - http://www.scopus.com/inward/record.url?scp=85045919915&partnerID=8YFLogxK
U2 - 10.1016/j.arcmed.2018.04.004
DO - 10.1016/j.arcmed.2018.04.004
M3 - Artículo
C2 - 29681412
SN - 0188-4409
VL - 49
SP - 27
EP - 35
JO - Archives of Medical Research
JF - Archives of Medical Research
IS - 1
ER -