TY - JOUR
T1 - Vitellogenin induction in the endangered goodeid fish Girardinichthys viviparus
T2 - Vitellogenin characterization and estrogenic effects of polychlorinated biphenyls
AU - Vega-López, Armando
AU - Martínez-Tabche, Laura
AU - Domínguez-López, Maria Lilia
AU - García-Latorre, Ethel
AU - Ramón-Gallegos, Eva
AU - García-Gasca, Alejandra
PY - 2006
Y1 - 2006
N2 - Vitellogenin (VTG) is a widely used biomarker in studies of endocrine disruption induced by xenobiotics such as polychlorinated biphenyls (PCBs). This study evaluates the estrogenic effects of these compounds on the black-fin goodeid Girardinichthys viviparus, an endangered fish species in Mexico with a reduced range of distribution due to pollution of its natural environment. Adult fish born in the laboratory were exposed to half the LC0 of Inerteen® commercial PCB mixture. VTG was determined through an inhibition enzyme-linked immunosorbent assay (ELISA) using a homologous-heterologous system. Male and female fish were killed after 1, 2, 4, 8 and 16 days of exposure. The distal third of each specimen was used for analysis. VTG was obtained from cultured hepatocytes and blood serum of males previously exposed to 17β-estradiol. VTG molecular mass was 348 kDa. PCBs were found to elicit greater estrogenic effects on VTG induction in males than in females (p < 0.05) and sex differences were noted. Time-dependent VTG induction kinetics in males and a stationary phase in females were also observed.
AB - Vitellogenin (VTG) is a widely used biomarker in studies of endocrine disruption induced by xenobiotics such as polychlorinated biphenyls (PCBs). This study evaluates the estrogenic effects of these compounds on the black-fin goodeid Girardinichthys viviparus, an endangered fish species in Mexico with a reduced range of distribution due to pollution of its natural environment. Adult fish born in the laboratory were exposed to half the LC0 of Inerteen® commercial PCB mixture. VTG was determined through an inhibition enzyme-linked immunosorbent assay (ELISA) using a homologous-heterologous system. Male and female fish were killed after 1, 2, 4, 8 and 16 days of exposure. The distal third of each specimen was used for analysis. VTG was obtained from cultured hepatocytes and blood serum of males previously exposed to 17β-estradiol. VTG molecular mass was 348 kDa. PCBs were found to elicit greater estrogenic effects on VTG induction in males than in females (p < 0.05) and sex differences were noted. Time-dependent VTG induction kinetics in males and a stationary phase in females were also observed.
KW - Endocrine disruption
KW - Girardinichthys viviparus
KW - Goodeid fish
KW - PCBs
KW - Sex differences
KW - Vitellogenin
KW - Waterborne exposure
UR - http://www.scopus.com/inward/record.url?scp=33645013873&partnerID=8YFLogxK
U2 - 10.1016/j.cbpc.2005.11.009
DO - 10.1016/j.cbpc.2005.11.009
M3 - Artículo
SN - 1532-0456
VL - 142
SP - 356
EP - 364
JO - Comparative Biochemistry and Physiology - C Toxicology and Pharmacology
JF - Comparative Biochemistry and Physiology - C Toxicology and Pharmacology
IS - 3-4 SPEC. ISS.
ER -