TY - JOUR
T1 - VDR polymorphisms are associated with bone mineral density in post-menopausal Mayan-Mestizo women
AU - Canto-Cetina, Thelma
AU - Cetina Manzanilla, José Antonio
AU - González Herrera, Lizbeth
AU - Rojano-Mejía, David
AU - Coral-Vázquez, Ramón Mauricio
AU - Coronel, Agustín
AU - Canto, Patricia
N1 - Publisher Copyright:
© 2014 © 2014 Informa UK Ltd.
PY - 2015/9/3
Y1 - 2015/9/3
N2 - Background: Osteoporosis is characterized by low bone mineral density (BMD), which is determined by an interaction of genetic, metabolic and environmental factors.Aim: To analyse the association between two polymorphisms of VDR as well as their haplotypes with BMD in post-menopausal Maya-Mestizo women.Subjects and methods: This study comprised 600 post-menopausal Maya-Mestizo women. A structured questionnaire for risk factors was applied and BMD was assessed at the lumbar spine (LS) and total hip (TH) by dual-energy X-ray absorptiometry. DNA was extracted from blood leukocytes. Two single-nucleotide polymorphisms of VDR (rs731236 and rs2228570) were studied using real-time PCR allelic discrimination for genotyping. Differences between the means of the BMDs according to the genotype were analysed with covariance. Haplotype analysis was conducted.Results: TT genotype of rs731236 of VDR had higher BMD at total hip and femoral neck (FN), and one haplotype formed by the two polymorphisms was associated with only TH-BMD variations. This difference was statistically significant after adjustment for confounders. The genotype of rs2228570 of VDR analysis showed no significant differences with BMD variations.Conclusion: The results showed that the TT genotype of rs731236 of VDR and one haplotype formed by rs731236 and rs2228570 polymorphisms were associated with higher BMD at TH and FN.
AB - Background: Osteoporosis is characterized by low bone mineral density (BMD), which is determined by an interaction of genetic, metabolic and environmental factors.Aim: To analyse the association between two polymorphisms of VDR as well as their haplotypes with BMD in post-menopausal Maya-Mestizo women.Subjects and methods: This study comprised 600 post-menopausal Maya-Mestizo women. A structured questionnaire for risk factors was applied and BMD was assessed at the lumbar spine (LS) and total hip (TH) by dual-energy X-ray absorptiometry. DNA was extracted from blood leukocytes. Two single-nucleotide polymorphisms of VDR (rs731236 and rs2228570) were studied using real-time PCR allelic discrimination for genotyping. Differences between the means of the BMDs according to the genotype were analysed with covariance. Haplotype analysis was conducted.Results: TT genotype of rs731236 of VDR had higher BMD at total hip and femoral neck (FN), and one haplotype formed by the two polymorphisms was associated with only TH-BMD variations. This difference was statistically significant after adjustment for confounders. The genotype of rs2228570 of VDR analysis showed no significant differences with BMD variations.Conclusion: The results showed that the TT genotype of rs731236 of VDR and one haplotype formed by rs731236 and rs2228570 polymorphisms were associated with higher BMD at TH and FN.
KW - BMD
KW - VDR
KW - haplotypes
KW - polymorphisms
KW - post-menopausal Maya-Mestizo women
UR - http://www.scopus.com/inward/record.url?scp=84942867036&partnerID=8YFLogxK
U2 - 10.3109/03014460.2014.967295
DO - 10.3109/03014460.2014.967295
M3 - Artículo
C2 - 25347090
SN - 0301-4460
VL - 42
SP - 470
EP - 475
JO - Annals of Human Biology
JF - Annals of Human Biology
IS - 5
ER -