Variation in antioxidant properties and phenolics concentration in different organs of wild growing and greenhouse cultivated Castilleja tenuiflora Benth.

Alma R. López-Laredo, Yenny Adriana Gómez-Aguirre, Virginia Medina-Pérez, Guadalupe Salcedo-Morales, Gabriela Sepúlveda-Jiménez, Gabriela Trejo-Tapia

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Abstract

The content of total phenolic compounds and flavonoids was determined in methanol extracts of root, stem, leaves, and inflorescences from wild growing and greenhouse cultivated plants of Castilleja tenuiflora. The antioxidant activity in each extract was evaluated using three in vitro models: scavenging of free radicals with 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) diammonium salt (ABTS), and reducing power by the phosphomolybdenum assay. Both, antioxidant activity and phytochemicals content were influenced significantly (P < 0.05) by the source of the plant material and the organ. Cultivated plants had the highest content of phenolic compounds (37.95 mg gallic acid equiv. g-1dry weight, P < 0.05) and the strongest antioxidant activity. Total phenolic compounds content correlated significantly with the antioxidant activity for all studied plant material and organs (P < 0.05). TLC profile using DPPH as a detection reagent indicated that the phenylethanoids verbascoside and isoverbascoside are the main contributors to the free-radical scavenging of C. tenuiflora. Cultivated plants of C. tenuiflora are an alternative source of natural antioxidants to wild growing plants. The antioxidant properties of C. tenuiflora may be associated with its traditional use to treat conditions consistent with radical-related diseases (e.g. inflammation, tumors). © 2012 Franciszek Górski Institute of Plant Physiology, Polish Academy of Sciences, Kraków.
Original languageAmerican English
Pages (from-to)2435-2442
Number of pages8
JournalActa Physiologiae Plantarum
StatePublished - 1 Jan 2014

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Orobanchaceae
Castilleja
Antioxidants
greenhouses
antioxidants
antioxidant activity
phenolic compounds
Free Radicals
Plant Physiological Phenomena
verbascoside
Inflorescence
Gallic Acid
Sulfonic Acids
sulfonic acid
extracts
plant physiology
Phytochemicals
gallic acid
Flavonoids
biphenyl

Cite this

@article{e397aa3df36c4312a9473e76a6f16556,
title = "Variation in antioxidant properties and phenolics concentration in different organs of wild growing and greenhouse cultivated Castilleja tenuiflora Benth.",
abstract = "The content of total phenolic compounds and flavonoids was determined in methanol extracts of root, stem, leaves, and inflorescences from wild growing and greenhouse cultivated plants of Castilleja tenuiflora. The antioxidant activity in each extract was evaluated using three in vitro models: scavenging of free radicals with 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) diammonium salt (ABTS), and reducing power by the phosphomolybdenum assay. Both, antioxidant activity and phytochemicals content were influenced significantly (P < 0.05) by the source of the plant material and the organ. Cultivated plants had the highest content of phenolic compounds (37.95 mg gallic acid equiv. g-1dry weight, P < 0.05) and the strongest antioxidant activity. Total phenolic compounds content correlated significantly with the antioxidant activity for all studied plant material and organs (P < 0.05). TLC profile using DPPH as a detection reagent indicated that the phenylethanoids verbascoside and isoverbascoside are the main contributors to the free-radical scavenging of C. tenuiflora. Cultivated plants of C. tenuiflora are an alternative source of natural antioxidants to wild growing plants. The antioxidant properties of C. tenuiflora may be associated with its traditional use to treat conditions consistent with radical-related diseases (e.g. inflammation, tumors). {\circledC} 2012 Franciszek G{\'o}rski Institute of Plant Physiology, Polish Academy of Sciences, Krak{\'o}w.",
author = "L{\'o}pez-Laredo, {Alma R.} and G{\'o}mez-Aguirre, {Yenny Adriana} and Virginia Medina-P{\'e}rez and Guadalupe Salcedo-Morales and Gabriela Sep{\'u}lveda-Jim{\'e}nez and Gabriela Trejo-Tapia",
year = "2014",
month = "1",
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language = "American English",
pages = "2435--2442",
journal = "Acta Physiologiae Plantarum",
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Variation in antioxidant properties and phenolics concentration in different organs of wild growing and greenhouse cultivated Castilleja tenuiflora Benth. / López-Laredo, Alma R.; Gómez-Aguirre, Yenny Adriana; Medina-Pérez, Virginia; Salcedo-Morales, Guadalupe; Sepúlveda-Jiménez, Gabriela; Trejo-Tapia, Gabriela.

In: Acta Physiologiae Plantarum, 01.01.2014, p. 2435-2442.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Variation in antioxidant properties and phenolics concentration in different organs of wild growing and greenhouse cultivated Castilleja tenuiflora Benth.

AU - López-Laredo, Alma R.

AU - Gómez-Aguirre, Yenny Adriana

AU - Medina-Pérez, Virginia

AU - Salcedo-Morales, Guadalupe

AU - Sepúlveda-Jiménez, Gabriela

AU - Trejo-Tapia, Gabriela

PY - 2014/1/1

Y1 - 2014/1/1

N2 - The content of total phenolic compounds and flavonoids was determined in methanol extracts of root, stem, leaves, and inflorescences from wild growing and greenhouse cultivated plants of Castilleja tenuiflora. The antioxidant activity in each extract was evaluated using three in vitro models: scavenging of free radicals with 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) diammonium salt (ABTS), and reducing power by the phosphomolybdenum assay. Both, antioxidant activity and phytochemicals content were influenced significantly (P < 0.05) by the source of the plant material and the organ. Cultivated plants had the highest content of phenolic compounds (37.95 mg gallic acid equiv. g-1dry weight, P < 0.05) and the strongest antioxidant activity. Total phenolic compounds content correlated significantly with the antioxidant activity for all studied plant material and organs (P < 0.05). TLC profile using DPPH as a detection reagent indicated that the phenylethanoids verbascoside and isoverbascoside are the main contributors to the free-radical scavenging of C. tenuiflora. Cultivated plants of C. tenuiflora are an alternative source of natural antioxidants to wild growing plants. The antioxidant properties of C. tenuiflora may be associated with its traditional use to treat conditions consistent with radical-related diseases (e.g. inflammation, tumors). © 2012 Franciszek Górski Institute of Plant Physiology, Polish Academy of Sciences, Kraków.

AB - The content of total phenolic compounds and flavonoids was determined in methanol extracts of root, stem, leaves, and inflorescences from wild growing and greenhouse cultivated plants of Castilleja tenuiflora. The antioxidant activity in each extract was evaluated using three in vitro models: scavenging of free radicals with 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) diammonium salt (ABTS), and reducing power by the phosphomolybdenum assay. Both, antioxidant activity and phytochemicals content were influenced significantly (P < 0.05) by the source of the plant material and the organ. Cultivated plants had the highest content of phenolic compounds (37.95 mg gallic acid equiv. g-1dry weight, P < 0.05) and the strongest antioxidant activity. Total phenolic compounds content correlated significantly with the antioxidant activity for all studied plant material and organs (P < 0.05). TLC profile using DPPH as a detection reagent indicated that the phenylethanoids verbascoside and isoverbascoside are the main contributors to the free-radical scavenging of C. tenuiflora. Cultivated plants of C. tenuiflora are an alternative source of natural antioxidants to wild growing plants. The antioxidant properties of C. tenuiflora may be associated with its traditional use to treat conditions consistent with radical-related diseases (e.g. inflammation, tumors). © 2012 Franciszek Górski Institute of Plant Physiology, Polish Academy of Sciences, Kraków.

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EP - 2442

JO - Acta Physiologiae Plantarum

JF - Acta Physiologiae Plantarum

SN - 0137-5881

ER -