TY - JOUR
T1 - Use of ITS and ISSR markers in the molecular characterisation of Pleurotus djamor hybrid strains
AU - Aguilar Doroteo, Leticia
AU - Zárate Segura, Paola Berenice
AU - Villanueva Arce, Ramón
AU - Yáñez Fernández, Jorge
AU - Garín Aguilar, María Eugenia
AU - Guadarrama Mendoza, Paula Cecilia
AU - Valencia del Toro, Gustavo
N1 - Publisher Copyright:
© 2017 Asociación Española de Micología
PY - 2018/1
Y1 - 2018/1
N2 - Background: Molecular characterisation of wild type Pleurotus species is important for germplasm conservation and its further use for genetic improvement. No molecular studies have been performed with monokaryons used for producing hybrid strains, either with the reconstituted strains obtained by pairing those monokaryons. The molecular characterisation of parental dikaryons, hybrid, and reconstituted strains as well as monokaryotic strains, is therefore of utmost importance. Aims: To carry out the molecular identification of Pleurotus djamor strains, i.e. dikaryotic wild type strains, hybrid strains, and the monokaryotic strains used for the hybrid formation. Methods: Five wild type strains of P. djamor from different states in Mexico were collected and molecularly identified by sequencing the ITS1-5.8-ITS2 region using ITS1 and ITS4 universal oligonucleotides. Four hybrid strains were obtained by pairing neohaplonts of two wild type strains selected. Six ISSR markers were used for the molecular characterisation of monokaryotic and dikaryotic strains. Results: Using the ITS markers, an amplified product of 700 bp was obtained in five wild type strains, with a 99-100% similarity with P. djamor. A total of 95 fragments were obtained using the ISSR markers, with 99% of polymorphism. Conclusions: Wild type strains were identified as P. djamor, and were clearly grouped with Mexican strains from other states of Mexico. ISSR markers allowed the generation of polymorphic bands in monokaryotic and dikaryotic strains, splitting both types of strains. The high degree of polymorphism indicates the genetic diversity of P. djamor, an advantage in mushroom production and in the improving of the species.
AB - Background: Molecular characterisation of wild type Pleurotus species is important for germplasm conservation and its further use for genetic improvement. No molecular studies have been performed with monokaryons used for producing hybrid strains, either with the reconstituted strains obtained by pairing those monokaryons. The molecular characterisation of parental dikaryons, hybrid, and reconstituted strains as well as monokaryotic strains, is therefore of utmost importance. Aims: To carry out the molecular identification of Pleurotus djamor strains, i.e. dikaryotic wild type strains, hybrid strains, and the monokaryotic strains used for the hybrid formation. Methods: Five wild type strains of P. djamor from different states in Mexico were collected and molecularly identified by sequencing the ITS1-5.8-ITS2 region using ITS1 and ITS4 universal oligonucleotides. Four hybrid strains were obtained by pairing neohaplonts of two wild type strains selected. Six ISSR markers were used for the molecular characterisation of monokaryotic and dikaryotic strains. Results: Using the ITS markers, an amplified product of 700 bp was obtained in five wild type strains, with a 99-100% similarity with P. djamor. A total of 95 fragments were obtained using the ISSR markers, with 99% of polymorphism. Conclusions: Wild type strains were identified as P. djamor, and were clearly grouped with Mexican strains from other states of Mexico. ISSR markers allowed the generation of polymorphic bands in monokaryotic and dikaryotic strains, splitting both types of strains. The high degree of polymorphism indicates the genetic diversity of P. djamor, an advantage in mushroom production and in the improving of the species.
KW - Hybrids
KW - ISSR
KW - ITS
KW - Pleurotus
UR - http://www.scopus.com/inward/record.url?scp=85040579809&partnerID=8YFLogxK
U2 - 10.1016/j.riam.2017.06.003
DO - 10.1016/j.riam.2017.06.003
M3 - Artículo
C2 - 29361378
SN - 1130-1406
VL - 35
SP - 49
EP - 55
JO - Revista Iberoamericana de Micologia
JF - Revista Iberoamericana de Micologia
IS - 1
ER -