Use of AKR1C1 and TKTL1 in the diagnosis of low-grade squamous intraepithelial lesions from Mexican women

Alfonso Sequeda-Juárez; Adriana Jiménez; Araceli Espinosa-Montesinos; Maria Del Carmen Cardenas-Aguayo; Eva Ramón-Gallegos

doi:10.21873/anticanres.14648

Use of AKR1C1 and TKTL1 in the diagnosis of low-grade squamous intraepithelial lesions from Mexican women

Alfonso Sequeda-Juárez, Adriana Jiménez, Araceli Espinosa-Montesinos, Maria Del Carmen Cardenas-Aguayo, Eva Ramón-Gallegos

Escuela Nacional de Ciencias Biológicas (ENCB)

Research output: Contribution to journal › Article › peer-review

5 Scopus citations

Abstract

Background/Aim: To determine the differential protein profiles of cervical cancer cell lines in order to find potential targets that can be used as biomarkers in low-grade squamous intraepithelial lesions (LSIL) diagnosis. Materials and Methods: Proteomic analysis was performed on cervical cancer cell lines by 2D electrophoresis and liquid chromatography–mass spectrometry. Biomarker validation was performed in histological samples by immunofluorescence. Results: Aldo-keto reductase C1 (AKR1C1) and transketolase-like 1 (TKTL1) proteins were selected as biomarkers and their expression was increased in samples with LSIL diagnosis. TKTL1 in combination with AKR1C1 increased sensitivity and specificity to 75% and 66%, respectively, with an area under curve of 0.76 in receiver operating characteristics curve analysis. Conclusion: AKR1C1 and TKTL1 showed potential as biomarkers for diagnosis of LSIL in Mexican women, with similar sensitivity and specificity to the biomarkers used in clinical trials for diagnosis of LSIL.

Original language	English
Pages (from-to)	6273-6284
Number of pages	12
Journal	Anticancer Research
Volume	40
Issue number	11
DOIs	https://doi.org/10.21873/anticanres.14648
State	Published - Nov 2020

Keywords

Biomarker
Proteomics
Sensitivity
Specificity
Squamous intraepithelial lesion

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.21873/anticanres.14648

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title = "Use of AKR1C1 and TKTL1 in the diagnosis of low-grade squamous intraepithelial lesions from Mexican women",

abstract = "Background/Aim: To determine the differential protein profiles of cervical cancer cell lines in order to find potential targets that can be used as biomarkers in low-grade squamous intraepithelial lesions (LSIL) diagnosis. Materials and Methods: Proteomic analysis was performed on cervical cancer cell lines by 2D electrophoresis and liquid chromatography–mass spectrometry. Biomarker validation was performed in histological samples by immunofluorescence. Results: Aldo-keto reductase C1 (AKR1C1) and transketolase-like 1 (TKTL1) proteins were selected as biomarkers and their expression was increased in samples with LSIL diagnosis. TKTL1 in combination with AKR1C1 increased sensitivity and specificity to 75% and 66%, respectively, with an area under curve of 0.76 in receiver operating characteristics curve analysis. Conclusion: AKR1C1 and TKTL1 showed potential as biomarkers for diagnosis of LSIL in Mexican women, with similar sensitivity and specificity to the biomarkers used in clinical trials for diagnosis of LSIL.",

keywords = "Biomarker, Proteomics, Sensitivity, Specificity, Squamous intraepithelial lesion",

author = "Alfonso Sequeda-Ju{\'a}rez and Adriana Jim{\'e}nez and Araceli Espinosa-Montesinos and Cardenas-Aguayo, {Maria Del Carmen} and Eva Ram{\'o}n-Gallegos",

year = "2020",

month = nov,

doi = "10.21873/anticanres.14648",

language = "Ingl{\'e}s",

volume = "40",

pages = "6273--6284",

journal = "Anticancer Research",

issn = "0250-7005",

publisher = "International Institute of Anticancer Research",

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TY - JOUR

T1 - Use of AKR1C1 and TKTL1 in the diagnosis of low-grade squamous intraepithelial lesions from Mexican women

AU - Sequeda-Juárez, Alfonso

AU - Jiménez, Adriana

AU - Espinosa-Montesinos, Araceli

AU - Cardenas-Aguayo, Maria Del Carmen

AU - Ramón-Gallegos, Eva

PY - 2020/11

Y1 - 2020/11

N2 - Background/Aim: To determine the differential protein profiles of cervical cancer cell lines in order to find potential targets that can be used as biomarkers in low-grade squamous intraepithelial lesions (LSIL) diagnosis. Materials and Methods: Proteomic analysis was performed on cervical cancer cell lines by 2D electrophoresis and liquid chromatography–mass spectrometry. Biomarker validation was performed in histological samples by immunofluorescence. Results: Aldo-keto reductase C1 (AKR1C1) and transketolase-like 1 (TKTL1) proteins were selected as biomarkers and their expression was increased in samples with LSIL diagnosis. TKTL1 in combination with AKR1C1 increased sensitivity and specificity to 75% and 66%, respectively, with an area under curve of 0.76 in receiver operating characteristics curve analysis. Conclusion: AKR1C1 and TKTL1 showed potential as biomarkers for diagnosis of LSIL in Mexican women, with similar sensitivity and specificity to the biomarkers used in clinical trials for diagnosis of LSIL.

AB - Background/Aim: To determine the differential protein profiles of cervical cancer cell lines in order to find potential targets that can be used as biomarkers in low-grade squamous intraepithelial lesions (LSIL) diagnosis. Materials and Methods: Proteomic analysis was performed on cervical cancer cell lines by 2D electrophoresis and liquid chromatography–mass spectrometry. Biomarker validation was performed in histological samples by immunofluorescence. Results: Aldo-keto reductase C1 (AKR1C1) and transketolase-like 1 (TKTL1) proteins were selected as biomarkers and their expression was increased in samples with LSIL diagnosis. TKTL1 in combination with AKR1C1 increased sensitivity and specificity to 75% and 66%, respectively, with an area under curve of 0.76 in receiver operating characteristics curve analysis. Conclusion: AKR1C1 and TKTL1 showed potential as biomarkers for diagnosis of LSIL in Mexican women, with similar sensitivity and specificity to the biomarkers used in clinical trials for diagnosis of LSIL.

KW - Biomarker

KW - Proteomics

KW - Sensitivity

KW - Specificity

KW - Squamous intraepithelial lesion

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U2 - 10.21873/anticanres.14648

DO - 10.21873/anticanres.14648

M3 - Artículo

C2 - 33109565

AN - SCOPUS:85094831267

SN - 0250-7005

VL - 40

SP - 6273

EP - 6284

JO - Anticancer Research

JF - Anticancer Research

IS - 11

ER -

Use of AKR1C1 and TKTL1 in the diagnosis of low-grade squamous intraepithelial lesions from Mexican women

Abstract

Keywords

UN SDGs

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