TY - JOUR
T1 - Use of a recombinant envelope protein subunit antigen for specific serological diagnosis of West Nile virus infection
AU - Beasley, David W.C.
AU - Holbrook, Michael R.
AU - Travassos Da Rosa, Amelia P.A.
AU - Coffey, Lark
AU - Carrara, Anne Sophie
AU - Phillippi-Falkenstein, Kathrine
AU - Bohm, Rudolf P.
AU - Ratterree, Marion S.
AU - Lillibridge, Kristy M.
AU - Ludwig, George V.
AU - Estrada-Franco, Jose
AU - Weaver, Scott C.
AU - Tesh, Robert B.
AU - Shope, Robert E.
AU - Barrett, Alan D.T.
PY - 2004/6
Y1 - 2004/6
N2 - Serological diagnosis of West Nile virus (WNV) infection is complicated by extensive antigenic cross-reactivity with other closely related flaviviruses, such as St. Louis encephalitis virus. Here we describe a recombinant, bacterially expressed antigen equivalent to structural domain III of the WNV envelope protein that has allowed clear discrimination of antibody responses to WNV from those against other related flaviviruses in indirect enzyme-linked immunosorbent assays using standardized control antisera and field-collected samples.
AB - Serological diagnosis of West Nile virus (WNV) infection is complicated by extensive antigenic cross-reactivity with other closely related flaviviruses, such as St. Louis encephalitis virus. Here we describe a recombinant, bacterially expressed antigen equivalent to structural domain III of the WNV envelope protein that has allowed clear discrimination of antibody responses to WNV from those against other related flaviviruses in indirect enzyme-linked immunosorbent assays using standardized control antisera and field-collected samples.
UR - http://www.scopus.com/inward/record.url?scp=2942538171&partnerID=8YFLogxK
U2 - 10.1128/JCM.42.6.2759-2765.2004
DO - 10.1128/JCM.42.6.2759-2765.2004
M3 - Artículo
SN - 0095-1137
VL - 42
SP - 2759
EP - 2765
JO - Journal of Clinical Microbiology
JF - Journal of Clinical Microbiology
IS - 6
ER -