TY - JOUR
T1 - Urban PM2.5 induces ROS generation and RBC damage in COPD patients
AU - Torres-Ramos, Yessica D.
AU - Montoya-Estrada, Araceli
AU - Guzman-Grenfell, Alberto M.
AU - Mancilla-Ramirez, Javier
AU - Cardenas-Gonzalez, Beatriz
AU - Blanco-Jimenez, Salvador
AU - Sepulveda-Sanchez, Jose D.
AU - Ramirez-Venegas, Alejandra
AU - Hicks, Juan J.
PY - 2011/1/6
Y1 - 2011/1/6
N2 - Particulate matters (PM) produce adverse effects on the respiratory system and cause COPD. These effects are thought to involve intrinsic generation of ROS which are present in ambient PM (transition metals and aromatic organic compounds). Here, we examined the chemical composition and ultra-microscopic structure of PM2. 5. The effect of this PM was studied in red blood cell (RBC) membranes (ghosts) from healthy volunteers (n = 11) and COPD patients (n = 43). These effects were compared with that produced by a Fenton metal-catalytic ROS generator. Oxidative biomarkers and cell damage were singificantly increased in presence of PM2. 5 or ROS generator in RBC of COPD patients as compared with those in cells from healthy volunteers. In contrast, total SH groups, band 3 phospho-tyrosine phosphatase (PTPase) and glucose-6 phosphate dehydrogenase (G6PD) activities were all diminished in cells from COPD patients. In conclusion, PM2. 5 increases damage to RBCs from COPD patients, decreases the activity of PTPase and G6PD, and alters the function of the anionic exchanger (AE1) and the antioxidant response by decreasing SH groups.
AB - Particulate matters (PM) produce adverse effects on the respiratory system and cause COPD. These effects are thought to involve intrinsic generation of ROS which are present in ambient PM (transition metals and aromatic organic compounds). Here, we examined the chemical composition and ultra-microscopic structure of PM2. 5. The effect of this PM was studied in red blood cell (RBC) membranes (ghosts) from healthy volunteers (n = 11) and COPD patients (n = 43). These effects were compared with that produced by a Fenton metal-catalytic ROS generator. Oxidative biomarkers and cell damage were singificantly increased in presence of PM2. 5 or ROS generator in RBC of COPD patients as compared with those in cells from healthy volunteers. In contrast, total SH groups, band 3 phospho-tyrosine phosphatase (PTPase) and glucose-6 phosphate dehydrogenase (G6PD) activities were all diminished in cells from COPD patients. In conclusion, PM2. 5 increases damage to RBCs from COPD patients, decreases the activity of PTPase and G6PD, and alters the function of the anionic exchanger (AE1) and the antioxidant response by decreasing SH groups.
KW - Anionic exchanger
KW - COPD
KW - Erythrocyte
KW - Free radicals
KW - Glucose 6 phosphate dehydrogenase
KW - Hypoxia
KW - Oxidative stress
KW - PM
KW - Particulate matter
KW - Phosphotyrosine phosphatase
KW - Tissue
UR - http://www.scopus.com/inward/record.url?scp=80053597368&partnerID=8YFLogxK
M3 - Artículo
SN - 1945-0494
VL - 3 E
SP - 808
EP - 817
JO - Frontiers in Bioscience - Elite
JF - Frontiers in Bioscience - Elite
IS - 3
ER -