TY - JOUR
T1 - Unraveling the relevance of the polyadenylation factor EhCFIm25 in Entamoeba histolytica through proteomic analysis
AU - Salgado-Martínez, América Itzallana
AU - Avila-Bonilla, Rodolfo Gamaliel
AU - Ramírez-Moreno, Esther
AU - Castañón-Sánchez, Carlos Alberto
AU - López-Camarillo, César
AU - Marchat, Laurence A.
N1 - Publisher Copyright:
© 2021 The Authors. FEBS Open Bio published by John Wiley & Sons Ltd on behalf of Federation of European Biochemical Societies
PY - 2021/10
Y1 - 2021/10
N2 - We recently reported that silencing of the polyadenylation factor EhCFIm25 in Entamoeba histolytica, the protozoan which causes human amoebiasis, affects trophozoite proliferation, death, and virulence, suggesting that EhCFIm25 may have potential as a new biochemical target. Here, we performed a shotgun proteomic analysis to identify modulated proteins that could explain this phenotype. Data are available via ProteomeXchange with identifier PXD027784. Our results revealed changes in the abundance of 75 proteins. Interestingly, STRING analysis, functional GO-term annotations, KEGG analyses, and literature review showed that modulated proteins are mainly related to glycolysis and carbon metabolism, cytoskeleton dynamics, and parasite virulence, as well as gene expression and protein modifications. Further studies are needed to confirm the hypotheses emerging from this proteomic analysis, to thereby acquire a comprehensive view of the molecular mechanisms involved.
AB - We recently reported that silencing of the polyadenylation factor EhCFIm25 in Entamoeba histolytica, the protozoan which causes human amoebiasis, affects trophozoite proliferation, death, and virulence, suggesting that EhCFIm25 may have potential as a new biochemical target. Here, we performed a shotgun proteomic analysis to identify modulated proteins that could explain this phenotype. Data are available via ProteomeXchange with identifier PXD027784. Our results revealed changes in the abundance of 75 proteins. Interestingly, STRING analysis, functional GO-term annotations, KEGG analyses, and literature review showed that modulated proteins are mainly related to glycolysis and carbon metabolism, cytoskeleton dynamics, and parasite virulence, as well as gene expression and protein modifications. Further studies are needed to confirm the hypotheses emerging from this proteomic analysis, to thereby acquire a comprehensive view of the molecular mechanisms involved.
KW - CFIm25 silencing
KW - Entamoeba histolytica
KW - polyadenylation
KW - proteomics
KW - virulence
UR - http://www.scopus.com/inward/record.url?scp=85114736425&partnerID=8YFLogxK
U2 - 10.1002/2211-5463.13287
DO - 10.1002/2211-5463.13287
M3 - Artículo
C2 - 34486252
AN - SCOPUS:85114736425
SN - 2211-5463
VL - 11
SP - 2819
EP - 2835
JO - FEBS Open Bio
JF - FEBS Open Bio
IS - 10
ER -