TY - JOUR
T1 - Understanding the relationship between Mycobacterium bovis spoligotypes from cattle in Latin American Countries
AU - Zumárraga, M. J.
AU - Arriaga, C.
AU - Barandiaran, S.
AU - Cobos-Marín, L.
AU - de Waard, J.
AU - Estrada-Garcia, I.
AU - Figueiredo, T.
AU - Figueroa, A.
AU - Giménez, F.
AU - Gomes, H. M.
AU - Gonzalez-y-Merchand, J. A.
AU - Macías, A.
AU - Milián-Suazo, F.
AU - Rodríguez, C. A.R.
AU - Santillán, M. A.
AU - Suffys, P. N.
AU - Trangoni, M. D.
AU - Zárraga, A. M.
AU - Cataldi, A.
N1 - Funding Information:
This work was supported by INTA Grant AERG-232121 (Argentina), FONDOSAG C5-100-10-23 of the National Agriculture and Livestock Service; Chilean National Commission of Scientific Research and Technology, CONICYT, project codes FONDEF D02I1111 and FICR-EQU18 (Chile), and European Community, Grant No. HEALTH-F3-2008-200999. JAG-y-M and IE-G are fellows of COFAA, EDI, IPN (Mexico). We thank Carla Noli, Cecilia Romero Torres and Valeria Rocha for their excellent technical help. A.C. is a career member of CONICET, Argentina.
PY - 2013/2
Y1 - 2013/2
N2 - Spoligotyping is the most frequently used method for genotyping isolates of Mycobacterium bovis worldwide. In the current work, we compared spoligotypes from 1684 M. bovis isolates from Argentina (816), Brazil (412), Chile (66), Mexico (274) and Venezuela (116), obtained from cattle, humans, pigs, wild boars, farmed deer, goats, buffaloes, cats, and wild animals. A total of 269 different spoligotypes were found: 142 (8.4%) isolates presented orphan spoligotypes, whereas 1542 (91.6%) formed 113 different clusters. In cattle, SB0140 was the most representative spoligotype with 355 (24.6%) isolates, followed by SB0121 with 149 (10.3%) isolates. Clustering of spoligotypes ranged from 95.2% in Argentina to 85.3% in Mexico. Orphan spoligotypes were also variable, ranging from 23.7% in Mexico to 4.1% in Brazil. A large proportion of spoligotypes were common to the neighboring countries Argentina, Brazil and Chile. In conclusion, despite the diversity of spoligotypes found in the five countries studied, there are major patterns that predominate in these neighboring countries. These clusters may reflect a long-lasting active transmission of bovine tuberculosis or common historical origins of infection.
AB - Spoligotyping is the most frequently used method for genotyping isolates of Mycobacterium bovis worldwide. In the current work, we compared spoligotypes from 1684 M. bovis isolates from Argentina (816), Brazil (412), Chile (66), Mexico (274) and Venezuela (116), obtained from cattle, humans, pigs, wild boars, farmed deer, goats, buffaloes, cats, and wild animals. A total of 269 different spoligotypes were found: 142 (8.4%) isolates presented orphan spoligotypes, whereas 1542 (91.6%) formed 113 different clusters. In cattle, SB0140 was the most representative spoligotype with 355 (24.6%) isolates, followed by SB0121 with 149 (10.3%) isolates. Clustering of spoligotypes ranged from 95.2% in Argentina to 85.3% in Mexico. Orphan spoligotypes were also variable, ranging from 23.7% in Mexico to 4.1% in Brazil. A large proportion of spoligotypes were common to the neighboring countries Argentina, Brazil and Chile. In conclusion, despite the diversity of spoligotypes found in the five countries studied, there are major patterns that predominate in these neighboring countries. These clusters may reflect a long-lasting active transmission of bovine tuberculosis or common historical origins of infection.
KW - Bovine tuberculosis
KW - Clusters
KW - Mycobacterium bovis
KW - Spoligotyping
UR - http://www.scopus.com/inward/record.url?scp=84871486577&partnerID=8YFLogxK
U2 - 10.1016/j.rvsc.2012.07.012
DO - 10.1016/j.rvsc.2012.07.012
M3 - Artículo
SN - 0034-5288
VL - 94
SP - 9
EP - 21
JO - Research in Veterinary Science
JF - Research in Veterinary Science
IS - 1
ER -