Ultrastructural Changes on Entamoeba histolytica HM1-IMSS Caused by the Flavan-3-Ol, (-)-Epicatechin

Jacqueline Soto; Consuelo Gómez; Fernando Calzada; María Esther Ramírez

doi:10.1055/s-0029-1240599

Ultrastructural Changes on Entamoeba histolytica HM1-IMSS Caused by the Flavan-3-Ol, (-)-Epicatechin

Jacqueline Soto, Consuelo Gómez, Fernando Calzada, María Esther Ramírez

Escuela Nacional de Medicina y Homeopatía (ENMH)

Research output: Contribution to journal › Article › peer-review

12 Scopus citations

Abstract

The flavan-3-ol, (-)-epicatechin has been previously identified as the most important antiamoebic compound among the extracts from two medicinal plants: Rubus coriifolius and Geranium mexicanum. Here we report the effects of epicatechin on Entamoeba histolytica morphology, analyzed by electronic microscopy. E. histolytica trophozoites were incubated for 48h at 37°C in the presence of 1.9g/mL epicatechin and processed for electronic microscopy analysis. Epicatechin induced nuclear and cytoplasmic changes in the treated trophozoites. These morphological alterations are identical to the cellular changes experienced by E. histolytica trophozoites undergoing programmed cell death (PCD), suggesting that epicatechin could be an alternative compound to treat amoebiasis.

Original language	English
Pages (from-to)	611-612
Number of pages	2
Journal	Planta Medica
Volume	76
Issue number	6
DOIs	https://doi.org/10.1055/s-0029-1240599
State	Published - 2010

Keywords

(-)epicatechin
Electronic microscopy
Entamoeba histolytica
Flavan3ol
Geraniaceae
Geranium mexicanum
Morphology

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10.1055/s-0029-1240599

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title = "Ultrastructural Changes on Entamoeba histolytica HM1-IMSS Caused by the Flavan-3-Ol, (-)-Epicatechin",

abstract = "The flavan-3-ol, (-)-epicatechin has been previously identified as the most important antiamoebic compound among the extracts from two medicinal plants: Rubus coriifolius and Geranium mexicanum. Here we report the effects of epicatechin on Entamoeba histolytica morphology, analyzed by electronic microscopy. E. histolytica trophozoites were incubated for 48h at 37°C in the presence of 1.9g/mL epicatechin and processed for electronic microscopy analysis. Epicatechin induced nuclear and cytoplasmic changes in the treated trophozoites. These morphological alterations are identical to the cellular changes experienced by E. histolytica trophozoites undergoing programmed cell death (PCD), suggesting that epicatechin could be an alternative compound to treat amoebiasis.",

keywords = "(-)epicatechin, Electronic microscopy, Entamoeba histolytica, Flavan3ol, Geraniaceae, Geranium mexicanum, Morphology",

author = "Jacqueline Soto and Consuelo G{\'o}mez and Fernando Calzada and Ram{\'i}rez, {Mar{\'i}a Esther}",

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doi = "10.1055/s-0029-1240599",

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T1 - Ultrastructural Changes on Entamoeba histolytica HM1-IMSS Caused by the Flavan-3-Ol, (-)-Epicatechin

AU - Soto, Jacqueline

AU - Gómez, Consuelo

AU - Calzada, Fernando

AU - Ramírez, María Esther

PY - 2010

Y1 - 2010

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AB - The flavan-3-ol, (-)-epicatechin has been previously identified as the most important antiamoebic compound among the extracts from two medicinal plants: Rubus coriifolius and Geranium mexicanum. Here we report the effects of epicatechin on Entamoeba histolytica morphology, analyzed by electronic microscopy. E. histolytica trophozoites were incubated for 48h at 37°C in the presence of 1.9g/mL epicatechin and processed for electronic microscopy analysis. Epicatechin induced nuclear and cytoplasmic changes in the treated trophozoites. These morphological alterations are identical to the cellular changes experienced by E. histolytica trophozoites undergoing programmed cell death (PCD), suggesting that epicatechin could be an alternative compound to treat amoebiasis.

KW - (-)epicatechin

KW - Electronic microscopy

KW - Entamoeba histolytica

KW - Flavan3ol

KW - Geraniaceae

KW - Geranium mexicanum

KW - Morphology

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DO - 10.1055/s-0029-1240599

M3 - Artículo

SN - 0032-0943

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SP - 611

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JO - Planta Medica

JF - Planta Medica

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ER -

Ultrastructural Changes on Entamoeba histolytica HM1-IMSS Caused by the Flavan-3-Ol, (-)-Epicatechin

Abstract

Keywords

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