TY - JOUR
T1 - Ultrastructural changes on clinical isolates of trichophyton rubrum, trichophyton mentagrophytes, and microsporum gypseum caused by solanum chrysotrichum saponin SC-2
AU - López-Villegas, Edgar Oliver
AU - Herrera-Arellano, Armando
AU - De Los Ángeles Martínez-Rivera, María
AU - Álvarez, Laura
AU - Cano-Nepauseno, Magally
AU - Marquina, Silvia
AU - Rodríguez-Tovar, Aída Verónica
AU - Tortoriello, Jaime
PY - 2009
Y1 - 2009
N2 - Worldwide, dermatophytoses represent a high percentage of all superficial mycoses. The most frequently isolated dermatophyte is Trichophyton rubrum. Solanum chrysotrichum is a vegetal species widely used in Mexican traditional medicine to treat skin infections; its extract has been used to formulate an herbal medicinal product that is used successfully to treat tinea pedis. Spirostanic saponin SC-2 from S. chrysotrichum possesses high activity against dermatophytes. The present study reports the ultrastructural changes observed by means of transmission electron microscopy (TEM) in clinical isolates of T. rubrum, T. mentagrophytes, and Microsporum gypseum induced by saponin SC-2. Strains were grown in RPMI 1640 containing SC-2 (1600μg/mL). Fungi were harvested at 6, 12, 24, and 48h; controls without SC-2 were included. T. mentagrophytes was the most susceptible to the SC-2 saponin, followed by M.gypseum, while T. rubrum was the most resistant. The main alterations caused by the SC-2 saponin were as follows: i) loss of cytoplasmic membrane continuity; ii) organelle degradation; iii) to a lesser extent, irreversible damage to the fungal wall; and iv) cellular death.
AB - Worldwide, dermatophytoses represent a high percentage of all superficial mycoses. The most frequently isolated dermatophyte is Trichophyton rubrum. Solanum chrysotrichum is a vegetal species widely used in Mexican traditional medicine to treat skin infections; its extract has been used to formulate an herbal medicinal product that is used successfully to treat tinea pedis. Spirostanic saponin SC-2 from S. chrysotrichum possesses high activity against dermatophytes. The present study reports the ultrastructural changes observed by means of transmission electron microscopy (TEM) in clinical isolates of T. rubrum, T. mentagrophytes, and Microsporum gypseum induced by saponin SC-2. Strains were grown in RPMI 1640 containing SC-2 (1600μg/mL). Fungi were harvested at 6, 12, 24, and 48h; controls without SC-2 were included. T. mentagrophytes was the most susceptible to the SC-2 saponin, followed by M.gypseum, while T. rubrum was the most resistant. The main alterations caused by the SC-2 saponin were as follows: i) loss of cytoplasmic membrane continuity; ii) organelle degradation; iii) to a lesser extent, irreversible damage to the fungal wall; and iv) cellular death.
KW - Microsporum gypseum
KW - Solanaceae
KW - Solanum chrysotrichum
KW - Spirostanic saponin
KW - Trichophyton mentagrophytes
KW - Trichophyton rubrum
UR - http://www.scopus.com/inward/record.url?scp=73349087452&partnerID=8YFLogxK
U2 - 10.1055/s-0029-1185810
DO - 10.1055/s-0029-1185810
M3 - Artículo
C2 - 19551614
SN - 0032-0943
VL - 75
SP - 1517
EP - 1520
JO - Planta Medica
JF - Planta Medica
IS - 14
ER -