Tumor Targeting via Sialic Acid: [68Ga]DOTA-en-pba as a New Tool for Molecular Imaging of Cancer with PET

Rogelio Jimenez Juarez; Charalambos Tsoukalas; Simonetta Geninatti-Crich; Anastasios Gaitanis; Theodoros Tsotakos; Maria Paravatou-Petsotas; Silvio Aime; Constantinos D. Anagnostopoulos; Kristina Djanashvili; Penelope Bouziotis

doi:DOI: 10.1007/s11307-018-1176-0

Tumor Targeting via Sialic Acid: [68Ga]DOTA-en-pba as a New Tool for Molecular Imaging of Cancer with PET

Rogelio Jimenez Juarez, Charalambos Tsoukalas, Simonetta Geninatti-Crich, Anastasios Gaitanis, Theodoros Tsotakos, Maria Paravatou-Petsotas, Silvio Aime, Constantinos D. Anagnostopoulos, Kristina Djanashvili, Penelope Bouziotis

Escuela Nacional de Ciencias Biológicas (ENCB)

Research output: Contribution to journal › Article › peer-review

Abstract

The aim of this study was to demonstrate the potential of Ga-68-labeled macrocycle (DOTA-en-pba) conjugated with phenylboronic vector for tumor recognition by positron emission tomography (PET), based on targeting of the overexpressed sialic acid (Sia).
The imaging reporter DOTA-en-pba was synthesized and labeled with Ga-68 at high efficiency. Cell binding assay on Mel-C and B16-F10 melanoma cells was used to evaluate melanin production and Sia overexpression to determine the best model for demonstrating the capability of [68Ga]DOTA-en-pba to recognize tumors. The in vivo PET imaging was done with B16-F10 tumor-bearing SCID mice injected with [68Ga]DOTA-en- pba intravenously. Tumor, blood, and urine metabolites were assessed to evaluate the presence of a targeting agent.

Translated title of the contribution	Orientación tumoral mediante ácido siálico: [68Ga]DOTA-en-pba como nueva herramienta para la obtención de imágenes moleculares del cáncer con PETOrientación tumoral mediante ácido siálico: [68Ga]DOTA-en-pba como nueva herramienta para la obtención de imágenes moleculares del cáncer con PET
Original language	English
Article number	0
Pages (from-to)	1-10
Number of pages	10
Journal	Molecular Imaging and Biology
Volume	20
Issue number	0
DOIs	https://doi.org/DOI: 10.1007/s11307-018-1176-0
State	Published - 20 Feb 2018

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DOI: 10.1007/s11307-018-1176-0

Cite this

Jimenez Juarez, R., Tsoukalas, C., Geninatti-Crich, S., Gaitanis, A., Tsotakos, T., Paravatou-Petsotas, M., Aime, S., D. Anagnostopoulos, C., Djanashvili, K., & Bouziotis, P. (2018). Tumor Targeting via Sialic Acid: [68Ga]DOTA-en-pba as a New Tool for Molecular Imaging of Cancer with PET. Molecular Imaging and Biology, 20(0), 1-10. Article 0. https://doi.org/DOI: 10.1007/s11307-018-1176-0

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title = "Tumor Targeting via Sialic Acid: [68Ga]DOTA-en-pba as a New Tool for Molecular Imaging of Cancer with PET",

abstract = "The aim of this study was to demonstrate the potential of Ga-68-labeled macrocycle (DOTA-en-pba) conjugated with phenylboronic vector for tumor recognition by positron emission tomography (PET), based on targeting of the overexpressed sialic acid (Sia).The imaging reporter DOTA-en-pba was synthesized and labeled with Ga-68 at high efficiency. Cell binding assay on Mel-C and B16-F10 melanoma cells was used to evaluate melanin production and Sia overexpression to determine the best model for demonstrating the capability of [68Ga]DOTA-en-pba to recognize tumors. The in vivo PET imaging was done with B16-F10 tumor-bearing SCID mice injected with [68Ga]DOTA-en- pba intravenously. Tumor, blood, and urine metabolites were assessed to evaluate the presence of a targeting agent.",

keywords = "Sialic acid, Tumor recognition, Phenylboronic targeting vector, Gallium-68, PET imaging",

author = "{Jimenez Juarez}, Rogelio and Charalambos Tsoukalas and Simonetta Geninatti-Crich and Anastasios Gaitanis and Theodoros Tsotakos and Maria Paravatou-Petsotas and Silvio Aime and {D. Anagnostopoulos}, Constantinos and Kristina Djanashvili and Penelope Bouziotis",

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Jimenez Juarez, R, Tsoukalas, C, Geninatti-Crich, S, Gaitanis, A, Tsotakos, T, Paravatou-Petsotas, M, Aime, S, D. Anagnostopoulos, C, Djanashvili, K & Bouziotis, P 2018, 'Tumor Targeting via Sialic Acid: [68Ga]DOTA-en-pba as a New Tool for Molecular Imaging of Cancer with PET', Molecular Imaging and Biology, vol. 20, no. 0, 0, pp. 1-10. https://doi.org/DOI: 10.1007/s11307-018-1176-0

TY - JOUR

T1 - Tumor Targeting via Sialic Acid: [68Ga]DOTA-en-pba as a New Tool for Molecular Imaging of Cancer with PET

AU - Jimenez Juarez, Rogelio

AU - Tsoukalas, Charalambos

AU - Geninatti-Crich, Simonetta

AU - Gaitanis, Anastasios

AU - Tsotakos, Theodoros

AU - Paravatou-Petsotas, Maria

AU - Aime, Silvio

AU - D. Anagnostopoulos, Constantinos

AU - Djanashvili, Kristina

AU - Bouziotis, Penelope

PY - 2018/2/20

Y1 - 2018/2/20

N2 - The aim of this study was to demonstrate the potential of Ga-68-labeled macrocycle (DOTA-en-pba) conjugated with phenylboronic vector for tumor recognition by positron emission tomography (PET), based on targeting of the overexpressed sialic acid (Sia).The imaging reporter DOTA-en-pba was synthesized and labeled with Ga-68 at high efficiency. Cell binding assay on Mel-C and B16-F10 melanoma cells was used to evaluate melanin production and Sia overexpression to determine the best model for demonstrating the capability of [68Ga]DOTA-en-pba to recognize tumors. The in vivo PET imaging was done with B16-F10 tumor-bearing SCID mice injected with [68Ga]DOTA-en- pba intravenously. Tumor, blood, and urine metabolites were assessed to evaluate the presence of a targeting agent.

AB - The aim of this study was to demonstrate the potential of Ga-68-labeled macrocycle (DOTA-en-pba) conjugated with phenylboronic vector for tumor recognition by positron emission tomography (PET), based on targeting of the overexpressed sialic acid (Sia).The imaging reporter DOTA-en-pba was synthesized and labeled with Ga-68 at high efficiency. Cell binding assay on Mel-C and B16-F10 melanoma cells was used to evaluate melanin production and Sia overexpression to determine the best model for demonstrating the capability of [68Ga]DOTA-en-pba to recognize tumors. The in vivo PET imaging was done with B16-F10 tumor-bearing SCID mice injected with [68Ga]DOTA-en- pba intravenously. Tumor, blood, and urine metabolites were assessed to evaluate the presence of a targeting agent.

KW - Sialic acid, Tumor recognition, Phenylboronic targeting vector, Gallium-68, PET imaging

U2 - DOI: 10.1007/s11307-018-1176-0

DO - DOI: 10.1007/s11307-018-1176-0

M3 - Artículo

SN - 1536-1632

VL - 20

SP - 1

EP - 10

JO - Molecular Imaging and Biology

JF - Molecular Imaging and Biology

IS - 0

M1 - 0

ER -