TY - JOUR
T1 - Tumor necrosis factor-α -308G/A polymorphism is associated with active vitiligo vulgaris in a northeastern Mexican population
AU - Salinas-Santander, Mauricio
AU - Díaz-García, Daniel
AU - Rojas-Martínez, Augusto
AU - Cantú-Salinas, Cristina
AU - Sánchez-Domínguez, Celia
AU - Reyes-López, Miguel
AU - Cerda-Flores, Ricardo M.
AU - Ocampo-Candiani, Jorge
AU - Ortiz-López, Rocío
PY - 2012/5
Y1 - 2012/5
N2 - Vitiligo is a skin disease characterized by depigmentation. Its etiopathogenesis is unclear, but it has been associated with autoimmune processes. Gene polymorphisms in the tumor necrosis factor-α (TNF-α) have been associated with several imflammatory diseases. In particular, the -308G/A polymorphism in the gene promoter region has been reported to be associated with increased plasma levels of TNF-α and with an increased risk to develop autoimmune diseases. To date, this polymorphism has not been associated with vitiligo. To assess a possible association between the TNF-α -308G/A and vitiligo vulgaris (VV), 198 vitiligo patients and 395 control subjects were recruited for the study. A complete demographic and clinical profile of each case was registered to analyze the possible risk factors of vitiligo. Genomic DNA isolated from peripheral blood was subjected to PCR-RFLP for genotyping of the TNF-α -308G/A polymorphism. Causal associations were determined by χ 2 test and their respective OR was assessed in a 2x2 contingency table. When population variables of type of vitiligo, gender, age of disease onset, and active disease status were considered, an association between active VV and the TNF-α GA genotype was found (P=0.0295, OR=2.0; 95% CI 1.01-3.93). All other variables were irrelevant to vitiligo. Our data suggest a possible association between the TNF-α -308 GA genotype and the active form of VV in a Mexican population.
AB - Vitiligo is a skin disease characterized by depigmentation. Its etiopathogenesis is unclear, but it has been associated with autoimmune processes. Gene polymorphisms in the tumor necrosis factor-α (TNF-α) have been associated with several imflammatory diseases. In particular, the -308G/A polymorphism in the gene promoter region has been reported to be associated with increased plasma levels of TNF-α and with an increased risk to develop autoimmune diseases. To date, this polymorphism has not been associated with vitiligo. To assess a possible association between the TNF-α -308G/A and vitiligo vulgaris (VV), 198 vitiligo patients and 395 control subjects were recruited for the study. A complete demographic and clinical profile of each case was registered to analyze the possible risk factors of vitiligo. Genomic DNA isolated from peripheral blood was subjected to PCR-RFLP for genotyping of the TNF-α -308G/A polymorphism. Causal associations were determined by χ 2 test and their respective OR was assessed in a 2x2 contingency table. When population variables of type of vitiligo, gender, age of disease onset, and active disease status were considered, an association between active VV and the TNF-α GA genotype was found (P=0.0295, OR=2.0; 95% CI 1.01-3.93). All other variables were irrelevant to vitiligo. Our data suggest a possible association between the TNF-α -308 GA genotype and the active form of VV in a Mexican population.
KW - Active vitiligo vulgaris
KW - Autoimmune diseases
KW - Northeastern Mexican population
KW - PCR-RFLP
KW - Tumor necrosis factor-α -308G/A polymorphism
UR - http://www.scopus.com/inward/record.url?scp=84858650341&partnerID=8YFLogxK
U2 - 10.3892/etm.2012.508
DO - 10.3892/etm.2012.508
M3 - Artículo
AN - SCOPUS:84858650341
SN - 1792-0981
VL - 3
SP - 893
EP - 897
JO - Experimental and Therapeutic Medicine
JF - Experimental and Therapeutic Medicine
IS - 5
ER -