Trixis angustifolia hexanic extract displays  synergistic antibacterial activity against M. tuberculosis

Anahí C. Sánchez-Chávez; Anuar Salazar-Gómez; L. Gerardo Zepeda-Vallejo; María de L. Hernández de Jesús; Manuel Quintos-Escalante; Ma Elena Vargas-Díaz; Julieta Luna-Herrera

doi:10.1080/14786419.2017.1416381

Trixis angustifolia hexanic extract displays synergistic antibacterial activity against M. tuberculosis

Anahí C. Sánchez-Chávez, Anuar Salazar-Gómez, L. Gerardo Zepeda-Vallejo, María de L. Hernández de Jesús, Manuel Quintos-Escalante, Ma Elena Vargas-Díaz, Julieta Luna-Herrera

Escuela Nacional de Ciencias Biológicas (ENCB)

Research output: Contribution to journal › Article › peer-review

9 Scopus citations

Abstract

A phytochemical and antibacterial study of Trixisangustifolia, a species endemic to Mexico, was performed allowing the isolation of six flavones. The minimal inhibitory concentration (MIC) of the hexanic extract, against Mycobacterium tuberculosis H37Rv was 25 μg/mL. The hexanic extract caused a significant inhibition of intracellular mycobacterial growth at 12.5 μg/mL. The biodirected assay of hexane extract enabled the detection of an active fraction (AF) against M. tuberculosis (MIC = 12.5 μg/mL), and a major flavone 1 (pebrellin) with no antimycobacterial activity (MIC > 200 μg/mL). A subsequent combination antimicrobial assay showed a synergistic antimycobacterial effect of AF in combination with pebrellin; the results of the synergistic activity suggest that the antimycobacterial activity found in T. angustifolia is due to the combined action of diverse metabolites present in the plant.

Original language	English
Pages (from-to)	1477-1481
Number of pages	5
Journal	Natural Product Research
Volume	33
Issue number	10
DOIs	https://doi.org/10.1080/14786419.2017.1416381
State	Published - 19 May 2019

Keywords

Mycobacterium tuberculosis
Trixis angustifolia
flavones
synergism

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Access to Document

10.1080/14786419.2017.1416381

Cite this

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title = "Trixis angustifolia hexanic extract displays synergistic antibacterial activity against M. tuberculosis",

abstract = "A phytochemical and antibacterial study of Trixisangustifolia, a species endemic to Mexico, was performed allowing the isolation of six flavones. The minimal inhibitory concentration (MIC) of the hexanic extract, against Mycobacterium tuberculosis H37Rv was 25 μg/mL. The hexanic extract caused a significant inhibition of intracellular mycobacterial growth at 12.5 μg/mL. The biodirected assay of hexane extract enabled the detection of an active fraction (AF) against M. tuberculosis (MIC = 12.5 μg/mL), and a major flavone 1 (pebrellin) with no antimycobacterial activity (MIC > 200 μg/mL). A subsequent combination antimicrobial assay showed a synergistic antimycobacterial effect of AF in combination with pebrellin; the results of the synergistic activity suggest that the antimycobacterial activity found in T. angustifolia is due to the combined action of diverse metabolites present in the plant.",

keywords = "Mycobacterium tuberculosis, Trixis angustifolia, flavones, synergism",

author = "S{\'a}nchez-Ch{\'a}vez, {Anah{\'i} C.} and Anuar Salazar-G{\'o}mez and Zepeda-Vallejo, {L. Gerardo} and {Hern{\'a}ndez de Jes{\'u}s}, {Mar{\'i}a de L.} and Manuel Quintos-Escalante and Vargas-D{\'i}az, {Ma Elena} and Julieta Luna-Herrera",

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year = "2019",

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AU - Sánchez-Chávez, Anahí C.

AU - Salazar-Gómez, Anuar

AU - Zepeda-Vallejo, L. Gerardo

AU - Hernández de Jesús, María de L.

AU - Quintos-Escalante, Manuel

AU - Vargas-Díaz, Ma Elena

AU - Luna-Herrera, Julieta

PY - 2019/5/19

Y1 - 2019/5/19

N2 - A phytochemical and antibacterial study of Trixisangustifolia, a species endemic to Mexico, was performed allowing the isolation of six flavones. The minimal inhibitory concentration (MIC) of the hexanic extract, against Mycobacterium tuberculosis H37Rv was 25 μg/mL. The hexanic extract caused a significant inhibition of intracellular mycobacterial growth at 12.5 μg/mL. The biodirected assay of hexane extract enabled the detection of an active fraction (AF) against M. tuberculosis (MIC = 12.5 μg/mL), and a major flavone 1 (pebrellin) with no antimycobacterial activity (MIC > 200 μg/mL). A subsequent combination antimicrobial assay showed a synergistic antimycobacterial effect of AF in combination with pebrellin; the results of the synergistic activity suggest that the antimycobacterial activity found in T. angustifolia is due to the combined action of diverse metabolites present in the plant.

AB - A phytochemical and antibacterial study of Trixisangustifolia, a species endemic to Mexico, was performed allowing the isolation of six flavones. The minimal inhibitory concentration (MIC) of the hexanic extract, against Mycobacterium tuberculosis H37Rv was 25 μg/mL. The hexanic extract caused a significant inhibition of intracellular mycobacterial growth at 12.5 μg/mL. The biodirected assay of hexane extract enabled the detection of an active fraction (AF) against M. tuberculosis (MIC = 12.5 μg/mL), and a major flavone 1 (pebrellin) with no antimycobacterial activity (MIC > 200 μg/mL). A subsequent combination antimicrobial assay showed a synergistic antimycobacterial effect of AF in combination with pebrellin; the results of the synergistic activity suggest that the antimycobacterial activity found in T. angustifolia is due to the combined action of diverse metabolites present in the plant.

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