TY - JOUR
T1 - Trichoderma sp native from chili region of Poanas, Durango, Mexico antagonist against phytopathogen fungi
AU - Valencia, Gabriela Barragan
AU - Vargas, Vicente Hernandez
AU - Soto, Jose Natividad Uribe
AU - Jimenez, Nestor Naranjo
AU - Corral, Jesus Herrera
PY - 2011
Y1 - 2011
N2 - Problem statement: Presence of Trichoderma spp. in agricultural soils decrease incidence of diseases by phytopathogen fungi. Sanity diagnostic require to know if exist beneficial microorganism and what agricultural practices help to their propagation. Approach: Samples (30) were taken from soils and sick plants of ten sites in four localities of Valley of Poanas. Phytophthora capsici Leo, Rhizoctonia solani Kühn and Trichoderma sp were isolated in agar V8 and were identified by microscopy. Results: In the 30 samples analyzed the presence of Phytophthora capsici Leo and Rhizoctonia solani Kühn was determined. Two isolations of Trichoderma sp were obtained from soil, they had antagonist activity against to P. capsici and R. solani on agar-V8 medium and showed chitinase activity. Sugar production in chitinase (10 mg.mL-1) by crude extract of Trichoderma growth in basal medium more chitin was determined. The average of sugar production from strains were 0.1175 and 0.1125 mg.mL-1 and standard deviations were 0.0567 and 0.0567 in four repetition. Interviews were applied to fifty farmers about cultivars and cultivation practices. At least seven types of chili were cultivated in the region of the Valley of Poanas, inorganic fertilization, irrigation systems by channel, gates and pumps were used. One hundred percent of farmers reported diseases of Damping off and Phytophthora root. Biocides were not used to control these diseases. Conclusion: The natural presence of Trichoderma spp was detected in Valley of Poanas, but some practices as inorganic fertilization and irrigation system can be contributing to propagation of phytopathogen fungi.
AB - Problem statement: Presence of Trichoderma spp. in agricultural soils decrease incidence of diseases by phytopathogen fungi. Sanity diagnostic require to know if exist beneficial microorganism and what agricultural practices help to their propagation. Approach: Samples (30) were taken from soils and sick plants of ten sites in four localities of Valley of Poanas. Phytophthora capsici Leo, Rhizoctonia solani Kühn and Trichoderma sp were isolated in agar V8 and were identified by microscopy. Results: In the 30 samples analyzed the presence of Phytophthora capsici Leo and Rhizoctonia solani Kühn was determined. Two isolations of Trichoderma sp were obtained from soil, they had antagonist activity against to P. capsici and R. solani on agar-V8 medium and showed chitinase activity. Sugar production in chitinase (10 mg.mL-1) by crude extract of Trichoderma growth in basal medium more chitin was determined. The average of sugar production from strains were 0.1175 and 0.1125 mg.mL-1 and standard deviations were 0.0567 and 0.0567 in four repetition. Interviews were applied to fifty farmers about cultivars and cultivation practices. At least seven types of chili were cultivated in the region of the Valley of Poanas, inorganic fertilization, irrigation systems by channel, gates and pumps were used. One hundred percent of farmers reported diseases of Damping off and Phytophthora root. Biocides were not used to control these diseases. Conclusion: The natural presence of Trichoderma spp was detected in Valley of Poanas, but some practices as inorganic fertilization and irrigation system can be contributing to propagation of phytopathogen fungi.
KW - Antagonist activity
KW - Antagonist microorganism
KW - Capsicum annuum
KW - Chitinase activity
KW - Damping off
KW - Phytopathogen fungi
KW - Phytophthora root rot
KW - Sick plants
KW - Trichoderma spp
UR - http://www.scopus.com/inward/record.url?scp=79960269630&partnerID=8YFLogxK
U2 - 10.3844/ajabssp.2011.185.188
DO - 10.3844/ajabssp.2011.185.188
M3 - Artículo
AN - SCOPUS:79960269630
SN - 1557-4989
VL - 6
SP - 185
EP - 188
JO - American Journal of Agricultural and Biological Science
JF - American Journal of Agricultural and Biological Science
IS - 2
ER -