TY - JOUR
T1 - Trehalose accumulation in Saccharomyces cerevisiae cells
T2 - Experimental data and structured modeling
AU - Aranda, Juan S.
AU - Salgado, Edgar
AU - Taillandier, Patricia
N1 - Funding Information:
The authors wish to thank the National Council of Science and Technology (CONACyT) of Mexico, the National Polytechnic Institute (IPN) of Mexico and the National Council for Scientific Research (CNRS) of France for financial support.
PY - 2004/2
Y1 - 2004/2
N2 - Several studies have shown that the reserve disaccharide trehalose also protects yeast cells under environmental stress. Besides, it is acknowledged that trehalose improves yeast viability so its synthesis has become paramount in baker's yeast industry, as well as in wine and beer production. In this work, starving carbon or nitrogen conditions were imposed to Saccharomyces cerevisiae growing in fed-batch cultivations in order to induce biosynthesis and accumulation of trehalose. Our experimental results show that yeast can accumulate trehalose up to 13% of biomass dry weight (0.13gtrehalosegbiomass-1) under carbon or nitrogen starvation. A compartmentalized and biochemically structured model is proposed in order to mathematically describe these experimental findings. The model predictions were compared with literature results obtained under growth conditions different from those established in our experiments, resulting in a very good agreement and thus indicating that the proposed hypotheses are essentially correct.
AB - Several studies have shown that the reserve disaccharide trehalose also protects yeast cells under environmental stress. Besides, it is acknowledged that trehalose improves yeast viability so its synthesis has become paramount in baker's yeast industry, as well as in wine and beer production. In this work, starving carbon or nitrogen conditions were imposed to Saccharomyces cerevisiae growing in fed-batch cultivations in order to induce biosynthesis and accumulation of trehalose. Our experimental results show that yeast can accumulate trehalose up to 13% of biomass dry weight (0.13gtrehalosegbiomass-1) under carbon or nitrogen starvation. A compartmentalized and biochemically structured model is proposed in order to mathematically describe these experimental findings. The model predictions were compared with literature results obtained under growth conditions different from those established in our experiments, resulting in a very good agreement and thus indicating that the proposed hypotheses are essentially correct.
KW - Fed-batch cultivation
KW - Saccharomyces cerevisiae
KW - Trehalose
UR - http://www.scopus.com/inward/record.url?scp=1042298141&partnerID=8YFLogxK
U2 - 10.1016/S1369-703X(03)00148-7
DO - 10.1016/S1369-703X(03)00148-7
M3 - Artículo
SN - 1369-703X
VL - 17
SP - 129
EP - 140
JO - Biochemical Engineering Journal
JF - Biochemical Engineering Journal
IS - 2
ER -