Transformation of Medicago truncatula via infiltration of seedlings or flowering plants with Agrobacterium

Anthony T. Trieu; Stephen H. Burleigh; Igor V. Kardailsky; Ignacio E. Maldonado-Mendoza; Wayne K. Versaw; Laura A. Blaylock; Heungsop Shin; Tzyy Jen Chiou; Hiroaki Katagi; Gary R. Dewbre; Detlef Weigel; Maria J. Harrison

doi:10.1046/j.1365-313X.2000.00757.x

Transformation of Medicago truncatula via infiltration of seedlings or flowering plants with Agrobacterium

Anthony T. Trieu, Stephen H. Burleigh, Igor V. Kardailsky, Ignacio E. Maldonado-Mendoza, Wayne K. Versaw, Laura A. Blaylock, Heungsop Shin, Tzyy Jen Chiou, Hiroaki Katagi, Gary R. Dewbre, Detlef Weigel, Maria J. Harrison

Research output: Contribution to journal › Article › peer-review

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Abstract

Two rapid and simple in planta transformation methods have been developed for the model legume Medicago truncatula. The first approach is based on a method developed for transformation of Arabidopsis thaliana and involves infiltration of flowering plants with a suspension of Agrobacterium. The second method involves infiltration of young seedlings with Agrobacterium. In both cases a proportion of the progeny of the infiltrated plants is transformed. The transformation frequency ranges from 4.7 to 76% for the flower infiltration method, and from 2.9 to 27.6% for the seedling infiltration method. Both procedures resulted in a mixture of independent transformants and sibling transformants. The transformants were genetically stable, and analysis of the T₂ generation indicates that the transgenes are inherited in a Mendelian fashion. These transformation systems will increase the utility of M. truncatula as a model system and enable large-scale insertional mutagenesis. T-DNA tagging and the many adaptations of this approach provide a wide range of opportunities for the analysis of the unique aspects of legumes.

Original language	English
Pages (from-to)	531-541
Number of pages	11
Journal	Plant Journal
Volume	22
Issue number	6
DOIs	https://doi.org/10.1046/j.1365-313X.2000.00757.x
State	Published - Jun 2000
Externally published	Yes

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Trieu, A. T., Burleigh, S. H., Kardailsky, I. V., Maldonado-Mendoza, I. E., Versaw, W. K., Blaylock, L. A., Shin, H., Chiou, T. J., Katagi, H., Dewbre, G. R., Weigel, D., & Harrison, M. J. (2000). Transformation of Medicago truncatula via infiltration of seedlings or flowering plants with Agrobacterium. Plant Journal, 22(6), 531-541. https://doi.org/10.1046/j.1365-313X.2000.00757.x

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title = "Transformation of Medicago truncatula via infiltration of seedlings or flowering plants with Agrobacterium",

abstract = "Two rapid and simple in planta transformation methods have been developed for the model legume Medicago truncatula. The first approach is based on a method developed for transformation of Arabidopsis thaliana and involves infiltration of flowering plants with a suspension of Agrobacterium. The second method involves infiltration of young seedlings with Agrobacterium. In both cases a proportion of the progeny of the infiltrated plants is transformed. The transformation frequency ranges from 4.7 to 76% for the flower infiltration method, and from 2.9 to 27.6% for the seedling infiltration method. Both procedures resulted in a mixture of independent transformants and sibling transformants. The transformants were genetically stable, and analysis of the T2 generation indicates that the transgenes are inherited in a Mendelian fashion. These transformation systems will increase the utility of M. truncatula as a model system and enable large-scale insertional mutagenesis. T-DNA tagging and the many adaptations of this approach provide a wide range of opportunities for the analysis of the unique aspects of legumes.",

author = "Trieu, {Anthony T.} and Burleigh, {Stephen H.} and Kardailsky, {Igor V.} and Maldonado-Mendoza, {Ignacio E.} and Versaw, {Wayne K.} and Blaylock, {Laura A.} and Heungsop Shin and Chiou, {Tzyy Jen} and Hiroaki Katagi and Dewbre, {Gary R.} and Detlef Weigel and Harrison, {Maria J.}",

year = "2000",

month = jun,

doi = "10.1046/j.1365-313X.2000.00757.x",

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AU - Trieu, Anthony T.

AU - Burleigh, Stephen H.

AU - Kardailsky, Igor V.

AU - Maldonado-Mendoza, Ignacio E.

AU - Versaw, Wayne K.

AU - Blaylock, Laura A.

AU - Shin, Heungsop

AU - Chiou, Tzyy Jen

AU - Katagi, Hiroaki

AU - Dewbre, Gary R.

AU - Weigel, Detlef

AU - Harrison, Maria J.

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AB - Two rapid and simple in planta transformation methods have been developed for the model legume Medicago truncatula. The first approach is based on a method developed for transformation of Arabidopsis thaliana and involves infiltration of flowering plants with a suspension of Agrobacterium. The second method involves infiltration of young seedlings with Agrobacterium. In both cases a proportion of the progeny of the infiltrated plants is transformed. The transformation frequency ranges from 4.7 to 76% for the flower infiltration method, and from 2.9 to 27.6% for the seedling infiltration method. Both procedures resulted in a mixture of independent transformants and sibling transformants. The transformants were genetically stable, and analysis of the T2 generation indicates that the transgenes are inherited in a Mendelian fashion. These transformation systems will increase the utility of M. truncatula as a model system and enable large-scale insertional mutagenesis. T-DNA tagging and the many adaptations of this approach provide a wide range of opportunities for the analysis of the unique aspects of legumes.

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DO - 10.1046/j.1365-313X.2000.00757.x

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VL - 22

SP - 531

EP - 541

JO - Plant Journal

JF - Plant Journal

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Transformation of Medicago truncatula via infiltration of seedlings or flowering plants with Agrobacterium

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