TY - JOUR
T1 - Transcript profiling of Zea mays roots reveals gene responses to phosphate deficiency at the plant- and species-specific levels
AU - Calderon-Vazquez, Carlos
AU - Ibarra-Laclette, Enrique
AU - Caballero-Perez, Juan
AU - Herrera-Estrella, Luis
N1 - Funding Information:
We would like to thank June Simpson and José Lopez-Bucio for valuable help in reviewing the manuscript. We also thank Enrique Ramirez-Chavez for lipid analyses; Susana ML Fuentes-Guerra and Flor MX Zamudio-Hernandez for qRT-PCR analysis; Liu Jia from TIGR for microarray image analysis; EMBRAPA for maize lines; David Galbraith, Vicki Chandler, and Jack Gardiner at the BIO5 Institute at the University of Arizona who produced the arrays by the Microarray Resources for Maize Research Project supported by NSF DBI 0321663. Cheryl Vanier and J Burgueño for statistical advice. This work was supported in part by grants from SAGARPA (Zea-2006) and HHMI (Grant 55003677) to LH-E.
PY - 2008/6
Y1 - 2008/6
N2 - Maize (Zea mays) is the most widely cultivated crop around the world; however, it is commonly affected by phosphate (Pi) deficiency in many regions, particularly in acid and alkaline soils of developing countries. To cope with Pi deficiency, plants have evolved a large number of developmental and biochemical adaptations; however, for maize, the underlying molecular basis of these responses is still unknown. In this work, the transcriptional response of maize roots to Pi starvation at 1, 3, 6, and 10 d after the onset of Pi deprivation was assessed. The investigation revealed a total of 1179 Pi-responsive genes, of which 820 and 363 genes were found to be either up- or down-regulated, respectively, by 2-fold or more. Pi-responsive genes were found to be involved in various metabolic, signal transduction, and developmental gene networks. A large set of transcription factors, which may be potential targets for crop breeding, was identified. In addition, gene expression profiles and changes in specific metabolites were also correlated. The results show that several dicotyledonous plant responses to Pi starvation are conserved in maize, but that some genetic responses appear to be more specific and that Pi deficiency leads to a shift in the recycling of internal Pi in maize roots. Ultimately, this work provides a more comprehensive view of Pi-responses in a model for economically important cereals and also sets a framework to produce Pi-specific maize microarrays to study the changes in global gene expression between Pi-efficient and Pi-inefficient maize genotypes.
AB - Maize (Zea mays) is the most widely cultivated crop around the world; however, it is commonly affected by phosphate (Pi) deficiency in many regions, particularly in acid and alkaline soils of developing countries. To cope with Pi deficiency, plants have evolved a large number of developmental and biochemical adaptations; however, for maize, the underlying molecular basis of these responses is still unknown. In this work, the transcriptional response of maize roots to Pi starvation at 1, 3, 6, and 10 d after the onset of Pi deprivation was assessed. The investigation revealed a total of 1179 Pi-responsive genes, of which 820 and 363 genes were found to be either up- or down-regulated, respectively, by 2-fold or more. Pi-responsive genes were found to be involved in various metabolic, signal transduction, and developmental gene networks. A large set of transcription factors, which may be potential targets for crop breeding, was identified. In addition, gene expression profiles and changes in specific metabolites were also correlated. The results show that several dicotyledonous plant responses to Pi starvation are conserved in maize, but that some genetic responses appear to be more specific and that Pi deficiency leads to a shift in the recycling of internal Pi in maize roots. Ultimately, this work provides a more comprehensive view of Pi-responses in a model for economically important cereals and also sets a framework to produce Pi-specific maize microarrays to study the changes in global gene expression between Pi-efficient and Pi-inefficient maize genotypes.
KW - Abiotic stress
KW - Maize
KW - Microarrays
KW - Phosphate
KW - Root
UR - http://www.scopus.com/inward/record.url?scp=48649098577&partnerID=8YFLogxK
U2 - 10.1093/jxb/ern115
DO - 10.1093/jxb/ern115
M3 - Artículo
C2 - 18503042
AN - SCOPUS:48649098577
SN - 0022-0957
VL - 59
SP - 2479
EP - 2497
JO - Journal of Experimental Botany
JF - Journal of Experimental Botany
IS - 9
ER -