TY - JOUR
T1 - Towards the complete proteinaceous regulome of Acinetobacter baumannii
AU - Casella, Leila G.
AU - Weiss, Andy
AU - Pérez-Rueda, Ernesto
AU - Antonio Ibarra, J.
AU - Shaw, Lindsey N.
N1 - Publisher Copyright:
© 2017 The Authors.
PY - 2017/3
Y1 - 2017/3
N2 - The emergence of Acinetobacter baumannii strains, with broad multidrug-resistance phenotypes and novel virulence factors unique to hypervirulent strains, presents a major threat to human health worldwide. Although a number of studies have described virulence-affecting entities for this organism, very few have identified regulatory elements controlling their expression. Previously, our group has documented the global identification and curation of regulatory RNAs in A. baumannii. As such, in the present study, we detail an extension of this work, the performance of an extensive bioinformatic analysis to identify regulatory proteins in the recently annotated genome of the highly virulent AB5075 strain. In so doing, 243 transcription factors, 14 two-component systems (TCSs), 2 orphan response regulators, 1 hybrid TCS and 5 s factors were found. A comparison of these elements between AB5075 and other clinical isolates, as well as a laboratory strain, led to the identification of several conserved regulatory elements, whilst at the same time uncovering regulators unique to hypervirulent strains. Lastly, by comparing regulatory elements compiled in this study to genes shown to be essential for AB5075 infection, we were able to highlight elements with a specific importance for pathogenic behaviour. Collectively, our work offers a unique insight into the regulatory network of A. baumannii strains, and provides insight into the evolution of hypervirulent lineages.
AB - The emergence of Acinetobacter baumannii strains, with broad multidrug-resistance phenotypes and novel virulence factors unique to hypervirulent strains, presents a major threat to human health worldwide. Although a number of studies have described virulence-affecting entities for this organism, very few have identified regulatory elements controlling their expression. Previously, our group has documented the global identification and curation of regulatory RNAs in A. baumannii. As such, in the present study, we detail an extension of this work, the performance of an extensive bioinformatic analysis to identify regulatory proteins in the recently annotated genome of the highly virulent AB5075 strain. In so doing, 243 transcription factors, 14 two-component systems (TCSs), 2 orphan response regulators, 1 hybrid TCS and 5 s factors were found. A comparison of these elements between AB5075 and other clinical isolates, as well as a laboratory strain, led to the identification of several conserved regulatory elements, whilst at the same time uncovering regulators unique to hypervirulent strains. Lastly, by comparing regulatory elements compiled in this study to genes shown to be essential for AB5075 infection, we were able to highlight elements with a specific importance for pathogenic behaviour. Collectively, our work offers a unique insight into the regulatory network of A. baumannii strains, and provides insight into the evolution of hypervirulent lineages.
KW - Acinetobacter baumannii AB5075
KW - Genome comparison
KW - Sigma factors
KW - Transcription factors
KW - Two-component systems
UR - http://www.scopus.com/inward/record.url?scp=85061647038&partnerID=8YFLogxK
U2 - 10.1099/mgen.0.000107
DO - 10.1099/mgen.0.000107
M3 - Artículo
C2 - 28663824
AN - SCOPUS:85061647038
SN - 2057-5858
VL - 3
JO - Microbial Genomics
JF - Microbial Genomics
IS - 3
M1 - 000107
ER -