TY - JOUR
T1 - Tolerance to oxidative stress caused by copper (Cu) in Trichoderma asperellum To
AU - Ernesto Juniors, Pérez Torres
AU - Valeria, Camacho Luna
AU - Santiago, Pérez Ocampo
AU - Mario, Rodríguez Monroy
AU - Gabriela, Sepúlveda Jiménez
N1 - Publisher Copyright:
© 2020 Elsevier Ltd
PY - 2020/10
Y1 - 2020/10
N2 - The fungus Trichoderma asperellum isolate To is a potential agent for biological control of phytopathogens on onion (Allium cepa L.) crops and could be combined with Cu-based fungicides for use in integrated management strategies for onion diseases. However, Cu-based fungicides cause oxidative stress in fungi; antioxidant responses to oxidative stress caused by Cu have scarcely been documented in Trichoderma spp. The aim of this study was to evaluate tolerance to Cu and antioxidant responses to oxidative stress caused by Cu in T. asperellum isolate To, and in isolates Tt and Tm obtained from Solanum lycopersicum L. (tomato) and Mangifera indica L. (mango) crops, respectively. The T. asperellum isolates were exposed to different concentrations of two Cu sources in semi-solid culture medium (the fungicide Sure Gold® and CuSO4 × 5H2O [reagent grade]), or to 100 mg L−1of the two Cu sources in liquid culture medium. Isolate Tm had the highest tolerance index followed by isolates Tt and To. However, isolate To exhibited the highest accumulation of Cu in the mycelium and the least disruption of conidia production and lipid peroxidation levels, the latter related to an increase in catalase and peroxidase activity. These results indicate that catalases and peroxidases are involved in the tolerance of T. asperellum isolate To Cu and that this isolate could be used in combination with Cu-based fungicides for integrated management of onion diseases.
AB - The fungus Trichoderma asperellum isolate To is a potential agent for biological control of phytopathogens on onion (Allium cepa L.) crops and could be combined with Cu-based fungicides for use in integrated management strategies for onion diseases. However, Cu-based fungicides cause oxidative stress in fungi; antioxidant responses to oxidative stress caused by Cu have scarcely been documented in Trichoderma spp. The aim of this study was to evaluate tolerance to Cu and antioxidant responses to oxidative stress caused by Cu in T. asperellum isolate To, and in isolates Tt and Tm obtained from Solanum lycopersicum L. (tomato) and Mangifera indica L. (mango) crops, respectively. The T. asperellum isolates were exposed to different concentrations of two Cu sources in semi-solid culture medium (the fungicide Sure Gold® and CuSO4 × 5H2O [reagent grade]), or to 100 mg L−1of the two Cu sources in liquid culture medium. Isolate Tm had the highest tolerance index followed by isolates Tt and To. However, isolate To exhibited the highest accumulation of Cu in the mycelium and the least disruption of conidia production and lipid peroxidation levels, the latter related to an increase in catalase and peroxidase activity. These results indicate that catalases and peroxidases are involved in the tolerance of T. asperellum isolate To Cu and that this isolate could be used in combination with Cu-based fungicides for integrated management of onion diseases.
KW - Catalase
KW - Heavy metals
KW - Lipid peroxidation
KW - Onion
KW - Oxidative stress
KW - Peroxidase
UR - http://www.scopus.com/inward/record.url?scp=85090701692&partnerID=8YFLogxK
U2 - 10.1016/j.bcab.2020.101783
DO - 10.1016/j.bcab.2020.101783
M3 - Artículo
AN - SCOPUS:85090701692
SN - 1878-8181
VL - 29
JO - Biocatalysis and Agricultural Biotechnology
JF - Biocatalysis and Agricultural Biotechnology
M1 - 101783
ER -