TY - JOUR
T1 - TNFRSF11B gene haplotype and its association with bone mineral density variations in postmenopausal Mexican-Mestizo women
AU - Rojano-Mejía, David
AU - Coral-Vázquez, Ramón Mauricio
AU - Espinosa, Leticia Cortes
AU - Romero-Hidalgo, Sandra
AU - López-Medina, Guillermo
AU - García, María Del Carmen Aguirre
AU - Coronel, Agustín
AU - Ibarra, Roberto
AU - Canto, Patricia
N1 - Funding Information:
D. Rojano was supported by Consejo Nacional de Ciencia y Tecnología (CONACYT) and by a fellowship award from the Coordinación de Investigación en Salud, Instituto Mexicano del Seguro Social. G. López was supported by a fellowship award from the Instituto de Ciencia y Tecnología del Distrito Federal.
Funding Information:
This work was supported by a grant from the Instituto de Ciencia y Tecnología del Distrito Federal (#PICDS08-34, México).
PY - 2012/1
Y1 - 2012/1
N2 - Objective: Osteoporosis is a complex health disease characterized by low bone mineral density (BMD), which is determined by an interaction of genetics with metabolic and environmental factors. The tumor necrosis factor receptor superfamily, member 11b (TNFRSF11B) gene, has been investigated in relation to BMD. Three polymorphisms in/nearby TNFRSF11B have been associated with BMD variations in some populations. The aim of this study was to investigate the possible association among three SNPs of TNFRSF11B and their haplotypes with the presence of BMD variations in postmenopausal Mexican Mestizo women. Subjects and methods: One thousand unrelated postmenopausal women of Mexican-Mestizo ethnic origin, who attended the outpatient clinic for routine, general medical evaluation, were invited and 750 women accepted to participate in the study. A structured questionnaire for risk factors was applied and BMD was measured in total hip and lumbar spine by dual-energy X-ray absorptiometry. DNA was obtained from blood leukocytes. Three single-nucleotide polymorphisms in TNFRSF11B gene were studied: rs4355801, rs2073618, and rs6993813. Real-time PCR allelic discrimination was used for genotyping. Deviations from Hardy-Weinberg equilibrium were tested. Pairwise linkage disequilibrium between single nucleotide polymorphisms was calculated by direct correlation r 2, and haplotype analysis was conducted. Results: Of the subjects, 31% had osteoporosis, 45.1% had osteopenia, and 23.9% had normal BMD. Genotype and allele distributions showed no significant differences; however, A-G-T haplotype was associated with variations in femoral neck BMD (P = 0.022). Conclusions: In our study population, analysis of the haplotypes of TNFRSF11B is a better genetic marker for variations in BMD.
AB - Objective: Osteoporosis is a complex health disease characterized by low bone mineral density (BMD), which is determined by an interaction of genetics with metabolic and environmental factors. The tumor necrosis factor receptor superfamily, member 11b (TNFRSF11B) gene, has been investigated in relation to BMD. Three polymorphisms in/nearby TNFRSF11B have been associated with BMD variations in some populations. The aim of this study was to investigate the possible association among three SNPs of TNFRSF11B and their haplotypes with the presence of BMD variations in postmenopausal Mexican Mestizo women. Subjects and methods: One thousand unrelated postmenopausal women of Mexican-Mestizo ethnic origin, who attended the outpatient clinic for routine, general medical evaluation, were invited and 750 women accepted to participate in the study. A structured questionnaire for risk factors was applied and BMD was measured in total hip and lumbar spine by dual-energy X-ray absorptiometry. DNA was obtained from blood leukocytes. Three single-nucleotide polymorphisms in TNFRSF11B gene were studied: rs4355801, rs2073618, and rs6993813. Real-time PCR allelic discrimination was used for genotyping. Deviations from Hardy-Weinberg equilibrium were tested. Pairwise linkage disequilibrium between single nucleotide polymorphisms was calculated by direct correlation r 2, and haplotype analysis was conducted. Results: Of the subjects, 31% had osteoporosis, 45.1% had osteopenia, and 23.9% had normal BMD. Genotype and allele distributions showed no significant differences; however, A-G-T haplotype was associated with variations in femoral neck BMD (P = 0.022). Conclusions: In our study population, analysis of the haplotypes of TNFRSF11B is a better genetic marker for variations in BMD.
KW - Bone mineral density
KW - Postmenopausal Mexican-Mestizo women
KW - TNFRSF11B haplotypes
KW - TNFRSF11B polymorphisms
UR - http://www.scopus.com/inward/record.url?scp=84855439110&partnerID=8YFLogxK
U2 - 10.1016/j.maturitas.2011.10.009
DO - 10.1016/j.maturitas.2011.10.009
M3 - Artículo
C2 - 22079369
AN - SCOPUS:84855439110
SN - 0378-5122
VL - 71
SP - 49
EP - 54
JO - Maturitas
JF - Maturitas
IS - 1
ER -