TY - JOUR
T1 - Titanium dioxide nanoparticles inhibit proliferation and induce morphological changes and apoptosis in glial cells
AU - Márquez-Ramírez, Sandra Gissela
AU - Delgado-Buenrostro, Norma Laura
AU - Chirino, Yolanda Irasema
AU - Iglesias, Gisela Gutiérrez
AU - López-Marure, Rebeca
N1 - Funding Information:
We thank Dr. María Luisa Escobar from the Departamento de Biología Celular, Facultad de Ciencias, UNAM, Mexico, for her help in the analysis of samples with the fluorescence microscope. Our thanks is given to MS Elizabeth Soria Castro from the Departamento de Patología, Instituto Nacional de Cardiología “Ignacio Chávez”, Mexico, for the analysis of TiO 2 NPs by SEM. We also thank Dr. Gerardo Cabañas Moreno from the Centro de Nanociencias y Micro y Nanotecnologías, IPN, Mexico, who performed the characterization of TiO 2 nanoparticles. Sandra Gissela Marquez, graduate student from the Posgrado en Investigación en Medicina, Escuela Superior de Medicina from the Instituto Politécnico Nacional, and member of the Academia de Bioquimica Medica I, was supported by a CONACyT scholarship number 175962. We thank IACOD IA202611-1, and PAPCA 2010–2011 (Project number 27) for their support to this work.
PY - 2012
Y1 - 2012
N2 - Titanium dioxide nanoparticles (TiO2 NPs) are widely used in the chemical, electrical and electronic industries. TiO2 NPs can enter directly into the brain through the olfactory bulb and be deposited in the hippocampus region. We determined the effect of TiO2 NPs on rat and human glial cells, C6 and U373, respectively. We evaluated proliferation by crystal violet staining, internalization of TiO2 NPs, and cellular morphology by TEM analysis, as well as F-actin distribution by immunostaining and cell death by detecting active caspase-3 and DNA fragmentation. TiO2 NPs inhibited proliferation and induced morphological changes that were related with a decrease in immuno-location of F-actin fibers. TiO2 NPs were internalized and formation of vesicles was observed. TiO2 NPs induced apoptosis after 96 h of treatment. Hence, TiO2 NPs had a cytotoxic effect on glial cells, suggesting that exposure to TiO2 NPs could cause brain injury and be hazardous to health.
AB - Titanium dioxide nanoparticles (TiO2 NPs) are widely used in the chemical, electrical and electronic industries. TiO2 NPs can enter directly into the brain through the olfactory bulb and be deposited in the hippocampus region. We determined the effect of TiO2 NPs on rat and human glial cells, C6 and U373, respectively. We evaluated proliferation by crystal violet staining, internalization of TiO2 NPs, and cellular morphology by TEM analysis, as well as F-actin distribution by immunostaining and cell death by detecting active caspase-3 and DNA fragmentation. TiO2 NPs inhibited proliferation and induced morphological changes that were related with a decrease in immuno-location of F-actin fibers. TiO2 NPs were internalized and formation of vesicles was observed. TiO2 NPs induced apoptosis after 96 h of treatment. Hence, TiO2 NPs had a cytotoxic effect on glial cells, suggesting that exposure to TiO2 NPs could cause brain injury and be hazardous to health.
KW - Apoptosis
KW - Glial cells
KW - Nanoparticles
KW - Proliferation
KW - Titanium dioxide
UR - http://www.scopus.com/inward/record.url?scp=84868499831&partnerID=8YFLogxK
U2 - 10.1016/j.tox.2012.09.005
DO - 10.1016/j.tox.2012.09.005
M3 - Artículo
C2 - 23044362
SN - 0300-483X
VL - 302
SP - 146
EP - 156
JO - Toxicology
JF - Toxicology
IS - 2-3
ER -