Time-dependent perezone production in different culture systems of Acourtia cordata

Gabriela Gómez-Serrano, Eliseo Cristiani-Urbina, Thelma Lilia Villegas-Garrido

Research output: Contribution to journalArticle

Abstract

Perezone, a sesquiterpenic benzoquinone with diverse medicinal properties, accumulates in the roots of Acourtia species. In this time-dependent study, the production of perezone was followed in A. cordata culture systems of plants kept in vitro or acclimated and grown in pots. Perezone was characterized by several analytical methods, using the crystallized compound isolated from roots of wild plants as standard. A procedure was developed for its selective quantification, which considers the specific bathochromic shift of the absorbance band in the visible region between the spectra of perezone in its non-ionic and ionic forms, with intensity directly proportional to concentration. In vitro, perezone was recovered from A. cordata roots in average amounts of 5.21 mg g-1dry weight. Contrastingly, in plants under ex vitro conditions, perezone in roots increased logarithmically, rising from an average of 2.4 mg g-1dry weight at the 12thweek, to 43.6 mg g-1dry weight at the 31stweek, an amount comparable to wild plants. These findings show the feasibility of in vitro and ex vitro culture systems to propagate and conserve the germplasm of perezone-producing Acourtia plants, and a fast and reliable method for the quantification of this valuable compound. © 2012 Versita Warsaw and Springer-Verlag Wien.
Original languageAmerican English
Pages (from-to)507-518
Number of pages12
JournalCentral European Journal of Biology
DOIs
StatePublished - 1 Jun 2012

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wild plants
benzoquinones
medicinal properties
in vitro culture
absorbance
analytical methods
Weights and Measures
germplasm
Plant Roots
perezone
methodology
In Vitro Techniques

Cite this

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title = "Time-dependent perezone production in different culture systems of Acourtia cordata",
abstract = "Perezone, a sesquiterpenic benzoquinone with diverse medicinal properties, accumulates in the roots of Acourtia species. In this time-dependent study, the production of perezone was followed in A. cordata culture systems of plants kept in vitro or acclimated and grown in pots. Perezone was characterized by several analytical methods, using the crystallized compound isolated from roots of wild plants as standard. A procedure was developed for its selective quantification, which considers the specific bathochromic shift of the absorbance band in the visible region between the spectra of perezone in its non-ionic and ionic forms, with intensity directly proportional to concentration. In vitro, perezone was recovered from A. cordata roots in average amounts of 5.21 mg g-1dry weight. Contrastingly, in plants under ex vitro conditions, perezone in roots increased logarithmically, rising from an average of 2.4 mg g-1dry weight at the 12thweek, to 43.6 mg g-1dry weight at the 31stweek, an amount comparable to wild plants. These findings show the feasibility of in vitro and ex vitro culture systems to propagate and conserve the germplasm of perezone-producing Acourtia plants, and a fast and reliable method for the quantification of this valuable compound. {\circledC} 2012 Versita Warsaw and Springer-Verlag Wien.",
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Time-dependent perezone production in different culture systems of Acourtia cordata. / Gómez-Serrano, Gabriela; Cristiani-Urbina, Eliseo; Villegas-Garrido, Thelma Lilia.

In: Central European Journal of Biology, 01.06.2012, p. 507-518.

Research output: Contribution to journalArticle

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