TY - JOUR
T1 - Time-dependent perezone production in different culture systems of Acourtia cordata
AU - Gómez-Serrano, Gabriela
AU - Cristiani-Urbina, Eliseo
AU - Villegas-Garrido, Thelma Lilia
N1 - Funding Information:
The authors gratefully acknowledge the support provided by: the Polytechnic Environmental Program in the collects of wild plants and seeds, the scientific team of the Herbarium of the National School of Biological Sciences, National Polytechnic Institute, in the identification of the collected plants, the scientific team of the Central Laboratory of Spectroscopy at National School of Biological Sciences, National Polytechnic Institute, as well as the financial support provided by the Secretariat of Postgraduate Studies and Research, National Polytechnic Institute. E. Cristiani-Urbina and T.L. Villegas-Garrido are fellow holders of a grant from the Commission for Support and Operation of Academic Activities, National Polytechnic Institute, Mexico City, Mexico.
PY - 2012/6
Y1 - 2012/6
N2 - Perezone, a sesquiterpenic benzoquinone with diverse medicinal properties, accumulates in the roots of Acourtia species. In this time-dependent study, the production of perezone was followed in A. cordata culture systems of plants kept in vitro or acclimated and grown in pots. Perezone was characterized by several analytical methods, using the crystallized compound isolated from roots of wild plants as standard. A procedure was developed for its selective quantification, which considers the specific bathochromic shift of the absorbance band in the visible region between the spectra of perezone in its non-ionic and ionic forms, with intensity directly proportional to concentration. In vitro, perezone was recovered from A. cordata roots in average amounts of 5.21 mg g -1 dry weight. Contrastingly, in plants under ex vitro conditions, perezone in roots increased logarithmically, rising from an average of 2.4 mg g -1 dry weight at the 12 th week, to 43.6 mg g -1 dry weight at the 31 st week, an amount comparable to wild plants. These findings show the feasibility of in vitro and ex vitro culture systems to propagate and conserve the germplasm of perezone-producing Acourtia plants, and a fast and reliable method for the quantification of this valuable compound.
AB - Perezone, a sesquiterpenic benzoquinone with diverse medicinal properties, accumulates in the roots of Acourtia species. In this time-dependent study, the production of perezone was followed in A. cordata culture systems of plants kept in vitro or acclimated and grown in pots. Perezone was characterized by several analytical methods, using the crystallized compound isolated from roots of wild plants as standard. A procedure was developed for its selective quantification, which considers the specific bathochromic shift of the absorbance band in the visible region between the spectra of perezone in its non-ionic and ionic forms, with intensity directly proportional to concentration. In vitro, perezone was recovered from A. cordata roots in average amounts of 5.21 mg g -1 dry weight. Contrastingly, in plants under ex vitro conditions, perezone in roots increased logarithmically, rising from an average of 2.4 mg g -1 dry weight at the 12 th week, to 43.6 mg g -1 dry weight at the 31 st week, an amount comparable to wild plants. These findings show the feasibility of in vitro and ex vitro culture systems to propagate and conserve the germplasm of perezone-producing Acourtia plants, and a fast and reliable method for the quantification of this valuable compound.
KW - Acourtia cordata
KW - Perezone
KW - Plant cultures
KW - Selective quantification
KW - Sesquiterpene benzoquinone
UR - http://www.scopus.com/inward/record.url?scp=84859373347&partnerID=8YFLogxK
U2 - 10.2478/s11535-012-0035-2
DO - 10.2478/s11535-012-0035-2
M3 - Artículo
SN - 1895-104X
VL - 7
SP - 507
EP - 518
JO - Central European Journal of Biology
JF - Central European Journal of Biology
IS - 3
ER -