TY - JOUR
T1 - Thermal image of coffee-seed germ obtained by photoacoustic microscopy
AU - Domínguez-Pacheco, A.
AU - Hernández Aguilar, C.
AU - Cruz-Orea, Alfredo
AU - Isaac Alemán, E.
AU - Martínez Ortiz, E.
N1 - Funding Information:
Acknowledgments The authors thank Mexican agencies, CONACYT, COFAA, EDI, and SIP for financial support for the present research. We also acknowledge Ing. Esther Ayala, Ing. A.B. Soto, and Ing. M. Guerrero for their technical support in the Physics Department of CINVESTAV-IPN. One of the authors (A. Cruz-Orea) is grateful for partial financial support from CONACYT Project No. 103632.
PY - 2013/9
Y1 - 2013/9
N2 - Photoacoustic microscopy (PAM) has been shown to be a suitable technique to obtain thermal images of a wide variety of samples from semiconductors to biological material. In PAM, the incidence of a modulated laser beam on a sample within a photoacoustic (PA) cell, hermetically sealed, produces a PA signal which depends on the thermal and optical properties of the studied sample. By making a sweep of the modulated laser beam on the sample surface, it is possible to obtain the PA signal as a function of their x-y coordinates, and from this signal, it is possible to reconstruct thermal images of the sample. In this study, thermal images of a coffee-seed germ were obtained, with a difference of 12 h between them, by using the PAM technique. Thermal differences observed between images give information which reflects degradation due to the fact that germ cells undergo changes as a function of time. The thermal images obtained by the PAM technique could be applied to biological materials that have a complex constitution (not homogeneous) in their structures, and thermal differences can be observed. PAM is a non-destructive technique, which is an important feature for this type of study. Other applications of this technique can be performed in the agricultural and biotechnological areas.
AB - Photoacoustic microscopy (PAM) has been shown to be a suitable technique to obtain thermal images of a wide variety of samples from semiconductors to biological material. In PAM, the incidence of a modulated laser beam on a sample within a photoacoustic (PA) cell, hermetically sealed, produces a PA signal which depends on the thermal and optical properties of the studied sample. By making a sweep of the modulated laser beam on the sample surface, it is possible to obtain the PA signal as a function of their x-y coordinates, and from this signal, it is possible to reconstruct thermal images of the sample. In this study, thermal images of a coffee-seed germ were obtained, with a difference of 12 h between them, by using the PAM technique. Thermal differences observed between images give information which reflects degradation due to the fact that germ cells undergo changes as a function of time. The thermal images obtained by the PAM technique could be applied to biological materials that have a complex constitution (not homogeneous) in their structures, and thermal differences can be observed. PAM is a non-destructive technique, which is an important feature for this type of study. Other applications of this technique can be performed in the agricultural and biotechnological areas.
KW - Coffee-seed germ
KW - Photoacoustic microscopy
KW - Thermal image
UR - http://www.scopus.com/inward/record.url?scp=84886718982&partnerID=8YFLogxK
U2 - 10.1007/s10765-012-1331-9
DO - 10.1007/s10765-012-1331-9
M3 - Artículo
SN - 0195-928X
VL - 34
SP - 1499
EP - 1503
JO - International Journal of Thermophysics
JF - International Journal of Thermophysics
IS - 8-9
ER -