TY - JOUR
T1 - The transcriptional activator of the bfp operon in EPEC (PerA) interacts with the RNA polymerase alpha subunit
AU - Lara-Ochoa, Cristina
AU - González-Lara, Fabiola
AU - Romero-González, Luis E.
AU - Jaramillo-Rodríguez, Juan B.
AU - Vázquez-Arellano, Sergio I.
AU - Medrano-López, Abraham
AU - Cedillo-Ramírez, Lilia
AU - Martínez-Laguna, Ygnacio
AU - Girón, Jorge A.
AU - Pérez-Rueda, Ernesto
AU - Puente, José Luis
AU - Ibarra, J. Antonio
N1 - Publisher Copyright:
© 2021, The Author(s).
PY - 2021/12
Y1 - 2021/12
N2 - Enteropathogenic E. coli virulence genes are under the control of various regulators, one of which is PerA, an AraC/XylS-like regulator. PerA directly promotes its own expression and that of the bfp operon encoding the genes involved in the biogenesis of the bundle-forming pilus (BFP); it also activates PerC expression, which in turn stimulates locus of enterocyte effacement (LEE) activation through the LEE-encoded regulator Ler. Monomeric PerA directly binds to the per and bfp regulatory regions; however, it is not known whether interactions between PerA and the RNA polymerase (RNAP) are needed to activate gene transcription as has been observed for other AraC-like regulators. Results showed that PerA interacts with the alpha subunit of the RNAP polymerase and that it is necessary for the genetic and phenotypic expression of bfpA. Furthermore, an in silico analysis shows that PerA might be interacting with specific alpha subunit amino acids residues highlighting the direction of future experiments.
AB - Enteropathogenic E. coli virulence genes are under the control of various regulators, one of which is PerA, an AraC/XylS-like regulator. PerA directly promotes its own expression and that of the bfp operon encoding the genes involved in the biogenesis of the bundle-forming pilus (BFP); it also activates PerC expression, which in turn stimulates locus of enterocyte effacement (LEE) activation through the LEE-encoded regulator Ler. Monomeric PerA directly binds to the per and bfp regulatory regions; however, it is not known whether interactions between PerA and the RNA polymerase (RNAP) are needed to activate gene transcription as has been observed for other AraC-like regulators. Results showed that PerA interacts with the alpha subunit of the RNAP polymerase and that it is necessary for the genetic and phenotypic expression of bfpA. Furthermore, an in silico analysis shows that PerA might be interacting with specific alpha subunit amino acids residues highlighting the direction of future experiments.
UR - http://www.scopus.com/inward/record.url?scp=85104562251&partnerID=8YFLogxK
U2 - 10.1038/s41598-021-87586-0
DO - 10.1038/s41598-021-87586-0
M3 - Artículo
C2 - 33879812
AN - SCOPUS:85104562251
SN - 2045-2322
VL - 11
JO - Scientific Reports
JF - Scientific Reports
IS - 1
M1 - 8541
ER -