TY - JOUR
T1 - The relationship between cyto-genotoxic damage and oxidative stress produced by emerging pollutants on a bioindicator organism (Allium cepa)
T2 - The carbamazepine case
AU - García-Medina, Sandra
AU - Galar-Martínez, Marcela
AU - Gómez-Oliván, Leobardo Manuel
AU - Torres-Bezaury, Rosalía María del Consuelo
AU - Islas-Flores, Hariz
AU - Gasca-Pérez, Eloy
N1 - Publisher Copyright:
© 2020 Elsevier Ltd
PY - 2020/8
Y1 - 2020/8
N2 - The carbamazepine (CBZ) is one of the most frequently detected anticonvulsant drugs in water bodies. Although there are reports of its ecotoxicological effects in the scientific literature, toxicity studies have not focused on establishing the mechanism by which CBZ produces its effect at environmentally relevant concentrations. The objective of this work was to evaluate cyto-genotoxicity and its relationship with oxidative stress produced by carbamazepine in the Allium cepa model. The cytotoxicity and genotoxicity, as well as the biomarkers of oxidative stress were analyzed in the roots of A. cepa, exposed to 1 and 31.36 μg L−1 after 2, 6, 12, 24, 48 and 72 h. The results show that genotoxic capacity of this drug in the roots of A. cepa is related to the generation of oxidative stress, in particular with production of hydroperoxides and oxidized proteins. Also, the cytotoxic effect has a high correlation with DNA damage. The results of the present study clearly indicate that bioassays with sensitive plants such as A. cepa are useful and complementary tools to evaluate the environmental impact of emerging contaminants.
AB - The carbamazepine (CBZ) is one of the most frequently detected anticonvulsant drugs in water bodies. Although there are reports of its ecotoxicological effects in the scientific literature, toxicity studies have not focused on establishing the mechanism by which CBZ produces its effect at environmentally relevant concentrations. The objective of this work was to evaluate cyto-genotoxicity and its relationship with oxidative stress produced by carbamazepine in the Allium cepa model. The cytotoxicity and genotoxicity, as well as the biomarkers of oxidative stress were analyzed in the roots of A. cepa, exposed to 1 and 31.36 μg L−1 after 2, 6, 12, 24, 48 and 72 h. The results show that genotoxic capacity of this drug in the roots of A. cepa is related to the generation of oxidative stress, in particular with production of hydroperoxides and oxidized proteins. Also, the cytotoxic effect has a high correlation with DNA damage. The results of the present study clearly indicate that bioassays with sensitive plants such as A. cepa are useful and complementary tools to evaluate the environmental impact of emerging contaminants.
KW - Antiepileptics
KW - Biomarkers of oxidative damage
KW - DNA damage
KW - Emerging contaminants
KW - Mitotic index
KW - Plant bioassays
UR - http://www.scopus.com/inward/record.url?scp=85082857045&partnerID=8YFLogxK
U2 - 10.1016/j.chemosphere.2020.126675
DO - 10.1016/j.chemosphere.2020.126675
M3 - Artículo
C2 - 32278918
AN - SCOPUS:85082857045
SN - 0045-6535
VL - 253
JO - Chemosphere
JF - Chemosphere
M1 - 126675
ER -