TY - JOUR
T1 - The potential of cold-shock promoters for the expression of recombinant proteins in microbes and mammalian cells
AU - Bartolo-Aguilar, Yaneth
AU - Chávez-Cabrera, Cipriano
AU - Flores-Cotera, Luis Bernardo
AU - Badillo-Corona, Jesús Agustín
AU - Oliver-Salvador, Carmen
AU - Marsch, Rodolfo
N1 - Publisher Copyright:
© 2022, The Author(s).
PY - 2022/12
Y1 - 2022/12
N2 - Background: Low-temperature expression of recombinant proteins may be advantageous to support their proper folding and preserve bioactivity. The generation of expression vectors regulated under cold conditions can improve the expression of some target proteins that are difficult to express in different expression systems. Main body of the abstract: The cspA encodes the major cold-shock protein from Escherichia coli (CspA). The promoter of cspA has been widely used to develop cold shock-inducible expression platforms in E. coli. Moreover, it is often necessary to employ expression systems other than bacteria, particularly when recombinant proteins require complex post-translational modifications. Currently, there are no commercial platforms available for expressing target genes by cold shock in eukaryotic cells. Consequently, genetic elements that respond to cold shock offer the possibility of developing novel cold-inducible expression platforms, particularly suitable for yeasts, and mammalian cells. Conclusions: This review covers the importance of the cellular response to low temperatures and the prospective use of cold-sensitive promoters to direct the expression of recombinant proteins. This concept may contribute to renewing interest in applying white technologies to produce recombinant proteins that are difficult to express. Graphical Abstract: [Figure not available: see fulltext.]
AB - Background: Low-temperature expression of recombinant proteins may be advantageous to support their proper folding and preserve bioactivity. The generation of expression vectors regulated under cold conditions can improve the expression of some target proteins that are difficult to express in different expression systems. Main body of the abstract: The cspA encodes the major cold-shock protein from Escherichia coli (CspA). The promoter of cspA has been widely used to develop cold shock-inducible expression platforms in E. coli. Moreover, it is often necessary to employ expression systems other than bacteria, particularly when recombinant proteins require complex post-translational modifications. Currently, there are no commercial platforms available for expressing target genes by cold shock in eukaryotic cells. Consequently, genetic elements that respond to cold shock offer the possibility of developing novel cold-inducible expression platforms, particularly suitable for yeasts, and mammalian cells. Conclusions: This review covers the importance of the cellular response to low temperatures and the prospective use of cold-sensitive promoters to direct the expression of recombinant proteins. This concept may contribute to renewing interest in applying white technologies to produce recombinant proteins that are difficult to express. Graphical Abstract: [Figure not available: see fulltext.]
KW - Active enzyme
KW - Aggregation-prone recombinant protein
KW - Cold-inducible promoter
KW - Protein quality
KW - Protein stability
KW - Unstable gene product
UR - http://www.scopus.com/inward/record.url?scp=85145422844&partnerID=8YFLogxK
U2 - 10.1186/s43141-022-00455-9
DO - 10.1186/s43141-022-00455-9
M3 - Artículo de revisión
C2 - 36580173
AN - SCOPUS:85145422844
SN - 1687-157X
VL - 20
JO - Journal of Genetic Engineering and Biotechnology
JF - Journal of Genetic Engineering and Biotechnology
IS - 1
M1 - 173
ER -