The flavonoid (-)-epicatechin affects cytoskeleton proteins and functions in Entamoeba histolytica

Verónica Bolaños, Alfredo Díaz-Martínez, Jacqueline Soto, Mario A. Rodríguez, Cesar López-Camarillo, Laurence A. Marchat, Esther Ramírez-Moreno

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Abstract

© 2014 Elsevier B.V. Human amoebiasis is an intestinal disease with a global distribution. Due to reports of parasite resistance or susceptibility reduction to metronidazole treatment, there is a renewed interest for the search of new molecules with antiamoebic activity. The flavonoid (-)-epicatechin that was isolated from the Mexican medicinal plant Geranium mexicanum HBK has an in vitro activity against E. histolytica trophozoites, however its molecular effects have been poorly documented. Using a proteomic approach based on two-dimensional gel electrophoresis and mass spectrometry (ESI-MS/MS) analysis, we evidenced that E. histolytica cytoskeleton proteins exhibit differential abundance in response to (-)-epicatechin treatment. Moreover, functional assays revealed modification on pathogenic mechanisms associated with cytoskeleton functionality, namely, adhesion, migration, phagocytosis and cytolysis. Consequently, these data suggested that (-)-epicatechin could affect virulence properties of this human pathogen. Biological significance: This work contributes with some advances in the action mechanisms involved in the antiamoebic effect of the flavonoid (-)-epicatechin. We found that this flavonoid has an unusual effect on trophozoites growth that is dependent of its concentration. Additionally, we reported that (-)-epicatechin affects mainly amebic cytoskeleton proteins, which results in alteration on important virulence mechanisms, like adhesion, migration, phagocytosis and cytolysis. This study provides new knowledge about a potential alternative therapy directed to the treatment of amoebiasis.This article is part of a Special Issue entitled: Proteomics, mass spectrometry and peptidomics, Cancun 2013. Guest Editors: César López-Camarillo, Victoria Pando-Robles and Bronwyn Jane Barkla.
Original languageAmerican English
Pages (from-to)74-85
Number of pages65
JournalJournal of Proteomics
DOIs
StatePublished - 5 Dec 2014

Fingerprint

Entamoeba histolytica
Catechin
Cytoskeleton
Flavonoids
Amebiasis
Trophozoites
Proteins
Phagocytosis
Proteomics
Mass spectrometry
Virulence
Adhesion
Geranium
Intestinal Diseases
Electrospray Ionization Mass Spectrometry
Victoria
Metronidazole
Electrophoresis, Gel, Two-Dimensional
Pathogens
Medicinal Plants

Cite this

Bolaños, Verónica ; Díaz-Martínez, Alfredo ; Soto, Jacqueline ; Rodríguez, Mario A. ; López-Camarillo, Cesar ; Marchat, Laurence A. ; Ramírez-Moreno, Esther. / The flavonoid (-)-epicatechin affects cytoskeleton proteins and functions in Entamoeba histolytica. In: Journal of Proteomics. 2014 ; pp. 74-85.
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The flavonoid (-)-epicatechin affects cytoskeleton proteins and functions in Entamoeba histolytica. / Bolaños, Verónica; Díaz-Martínez, Alfredo; Soto, Jacqueline; Rodríguez, Mario A.; López-Camarillo, Cesar; Marchat, Laurence A.; Ramírez-Moreno, Esther.

In: Journal of Proteomics, 05.12.2014, p. 74-85.

Research output: Contribution to journalArticleResearchpeer-review

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T1 - The flavonoid (-)-epicatechin affects cytoskeleton proteins and functions in Entamoeba histolytica

AU - Bolaños, Verónica

AU - Díaz-Martínez, Alfredo

AU - Soto, Jacqueline

AU - Rodríguez, Mario A.

AU - López-Camarillo, Cesar

AU - Marchat, Laurence A.

AU - Ramírez-Moreno, Esther

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