TY - JOUR
T1 - The effects of NG-nitro-L-arginine methyl ester on systolic pressure, diastolic pressure and pulse pressure according to the initial level of blood pressure
AU - Ruiz, Antonio
AU - López, Ruth M.
AU - Pérez, Teresa
AU - Castillo, Carlos
AU - Castillo, Enrique F.
PY - 2008/2
Y1 - 2008/2
N2 - The objective of this study was to re-examine whether the effect of the nitric oxide synthesis inhibitor, NG-nitro-l-arginine methyl ester (l-NAME), on blood pressure depends on peripheral vascular tone. The effects of l-NAME (10 mg/kg, i.v.) on diastolic blood pressure (DBP), systolic blood pressure (SBP), pulse pressure (PP) and heart rate (HR) were studied in pithed rats. Sal-pithed rats received 0.9% NaCl, 10 μl/kg/min. Vascular tone was step-wise increased with 3, 10 and 30 μg/kg/min intravenous phenylephrine infusion (LPhe-pithed, MPhe-pithed and HPhe-pithed rats respectively). l-NAME elicited vasopressor responses in all the animals studied. l-NAME increases in SBP and DBP in Sal-pithed rats were significantly smaller than the ones obtained in phenylephrine infused rats. The increases in DBP elicited by l-NAME were greater in LPhe-pithed rats compared with those of MPhe-pithed and HPhe-pithed rats (i.e. the step-wise rises in DBP obtained with phenylephrine were inversely related to the increases in DBP produced by l-NAME); however, the increases in SBP were similar between these experimental groups. The PP increased during l-NAME-induced pressor responses in phenylephrine-infused rats. l-NAME increases in PP showed the following order: Sal-pithed < LPhe-pithed < MPhe-pithed ≤ HPhe-pithed rats. HR was not modified by l-NAME. In conclusion, the vasopressor responses produced by l-NAME in pithed rats are influenced by the pre-existing vasomotor tone in complex form. We did not find a simple positive correlation between the vascular tone or level of arterial pressure, and the magnitude of the diastolic and systolic pressor responses elicited by l-NAME. Interestingly, the increase in PP induced by l-NAME was greater in accordance with the increasing value of baseline arterial pressure. NO synthesis inhibition in the arterial endothelium may possibly explain the increase in PP caused by l-NAME, as resulting from the reduction in proximal conduit artery compliance.
AB - The objective of this study was to re-examine whether the effect of the nitric oxide synthesis inhibitor, NG-nitro-l-arginine methyl ester (l-NAME), on blood pressure depends on peripheral vascular tone. The effects of l-NAME (10 mg/kg, i.v.) on diastolic blood pressure (DBP), systolic blood pressure (SBP), pulse pressure (PP) and heart rate (HR) were studied in pithed rats. Sal-pithed rats received 0.9% NaCl, 10 μl/kg/min. Vascular tone was step-wise increased with 3, 10 and 30 μg/kg/min intravenous phenylephrine infusion (LPhe-pithed, MPhe-pithed and HPhe-pithed rats respectively). l-NAME elicited vasopressor responses in all the animals studied. l-NAME increases in SBP and DBP in Sal-pithed rats were significantly smaller than the ones obtained in phenylephrine infused rats. The increases in DBP elicited by l-NAME were greater in LPhe-pithed rats compared with those of MPhe-pithed and HPhe-pithed rats (i.e. the step-wise rises in DBP obtained with phenylephrine were inversely related to the increases in DBP produced by l-NAME); however, the increases in SBP were similar between these experimental groups. The PP increased during l-NAME-induced pressor responses in phenylephrine-infused rats. l-NAME increases in PP showed the following order: Sal-pithed < LPhe-pithed < MPhe-pithed ≤ HPhe-pithed rats. HR was not modified by l-NAME. In conclusion, the vasopressor responses produced by l-NAME in pithed rats are influenced by the pre-existing vasomotor tone in complex form. We did not find a simple positive correlation between the vascular tone or level of arterial pressure, and the magnitude of the diastolic and systolic pressor responses elicited by l-NAME. Interestingly, the increase in PP induced by l-NAME was greater in accordance with the increasing value of baseline arterial pressure. NO synthesis inhibition in the arterial endothelium may possibly explain the increase in PP caused by l-NAME, as resulting from the reduction in proximal conduit artery compliance.
KW - L-NAME
KW - Nitric oxide
KW - Pithed rat
KW - Pulse pressure
UR - http://www.scopus.com/inward/record.url?scp=38649101850&partnerID=8YFLogxK
U2 - 10.1111/j.1472-8206.2007.00560.x
DO - 10.1111/j.1472-8206.2007.00560.x
M3 - Artículo
C2 - 18251721
SN - 0767-3981
VL - 22
SP - 45
EP - 52
JO - Fundamental and Clinical Pharmacology
JF - Fundamental and Clinical Pharmacology
IS - 1
ER -