Taking advantage of a high-throughput flow cytometer for the implementation of an ADCC assay for regulatory compliance

Rosa Camacho-Sandoval; Alexis Jiménez-Uribe; Alejandra V. Tenorio-Calvo; Carlos A. López-Morales; Leslie Muñoz-García; Alejandra Montes-Luna; Héctor Leonardo García-Xolalpa; Marco Velasco-Velázquez; Lenin Pavón; Sonia Mayra Pérez-Tapia; Emilio Medina-Rivero

doi:10.1016/j.btre.2020.e00456

Taking advantage of a high-throughput flow cytometer for the implementation of an ADCC assay for regulatory compliance

Rosa Camacho-Sandoval, Alexis Jiménez-Uribe, Alejandra V. Tenorio-Calvo, Carlos A. López-Morales, Leslie Muñoz-García, Alejandra Montes-Luna, Héctor Leonardo García-Xolalpa, Marco Velasco-Velázquez, Lenin Pavón, Sonia Mayra Pérez-Tapia, Emilio Medina-Rivero

Escuela Nacional de Ciencias Biológicas (ENCB)

Research output: Contribution to journal › Article › peer-review

Abstract

Technological advances allowed the development of high-throughput instruments such as IntelliCyt iQue Screener PLUS®. Here, we took advantage of this technology to transfer a previously validated cytotoxicity assay. The evaluated parameters were cell permeability, caspase activation and phosphatidyl serine exposure. The assay was accurate (r² = 0.90), precise (%CV ≤ 18.90) and specific. These results showed that this technology is suitable to be used in control quality environments. In addition, the automation provided a faster acquisition and analysis of data with precise and accurate results. This application could be implemented to evaluate another in vitro mechanism of action of different biotherapeutics.

Original language	English
Article number	e00456
Journal	Biotechnology Reports
Volume	26
DOIs	https://doi.org/10.1016/j.btre.2020.e00456
State	Published - Jun 2020

Keywords

ADCC
High-throughput flow cytometry
Validation bioassay

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10.1016/j.btre.2020.e00456

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Camacho-Sandoval, R., Jiménez-Uribe, A., Tenorio-Calvo, A. V., López-Morales, C. A., Muñoz-García, L., Montes-Luna, A., García-Xolalpa, H. L., Velasco-Velázquez, M., Pavón, L., Pérez-Tapia, S. M., & Medina-Rivero, E. (2020). Taking advantage of a high-throughput flow cytometer for the implementation of an ADCC assay for regulatory compliance. Biotechnology Reports, 26, Article e00456. https://doi.org/10.1016/j.btre.2020.e00456

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title = "Taking advantage of a high-throughput flow cytometer for the implementation of an ADCC assay for regulatory compliance",

abstract = "Technological advances allowed the development of high-throughput instruments such as IntelliCyt iQue Screener PLUS{\textregistered}. Here, we took advantage of this technology to transfer a previously validated cytotoxicity assay. The evaluated parameters were cell permeability, caspase activation and phosphatidyl serine exposure. The assay was accurate (r2 = 0.90), precise (%CV ≤ 18.90) and specific. These results showed that this technology is suitable to be used in control quality environments. In addition, the automation provided a faster acquisition and analysis of data with precise and accurate results. This application could be implemented to evaluate another in vitro mechanism of action of different biotherapeutics.",

keywords = "ADCC, High-throughput flow cytometry, Validation bioassay",

author = "Rosa Camacho-Sandoval and Alexis Jim{\'e}nez-Uribe and Tenorio-Calvo, {Alejandra V.} and L{\'o}pez-Morales, {Carlos A.} and Leslie Mu{\~n}oz-Garc{\'i}a and Alejandra Montes-Luna and Garc{\'i}a-Xolalpa, {H{\'e}ctor Leonardo} and Marco Velasco-Vel{\'a}zquez and Lenin Pav{\'o}n and P{\'e}rez-Tapia, {Sonia Mayra} and Emilio Medina-Rivero",

note = "Publisher Copyright: {\textcopyright} 2020 The Authors",

year = "2020",

month = jun,

doi = "10.1016/j.btre.2020.e00456",

language = "Ingl{\'e}s",

volume = "26",

journal = "Biotechnology Reports",

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Camacho-Sandoval, R, Jiménez-Uribe, A, Tenorio-Calvo, AV, López-Morales, CA, Muñoz-García, L, Montes-Luna, A, García-Xolalpa, HL, Velasco-Velázquez, M, Pavón, L, Pérez-Tapia, SM & Medina-Rivero, E 2020, 'Taking advantage of a high-throughput flow cytometer for the implementation of an ADCC assay for regulatory compliance', Biotechnology Reports, vol. 26, e00456. https://doi.org/10.1016/j.btre.2020.e00456

TY - JOUR

T1 - Taking advantage of a high-throughput flow cytometer for the implementation of an ADCC assay for regulatory compliance

AU - Camacho-Sandoval, Rosa

AU - Jiménez-Uribe, Alexis

AU - Tenorio-Calvo, Alejandra V.

AU - López-Morales, Carlos A.

AU - Muñoz-García, Leslie

AU - Montes-Luna, Alejandra

AU - García-Xolalpa, Héctor Leonardo

AU - Velasco-Velázquez, Marco

AU - Pavón, Lenin

AU - Pérez-Tapia, Sonia Mayra

AU - Medina-Rivero, Emilio

PY - 2020/6

Y1 - 2020/6

N2 - Technological advances allowed the development of high-throughput instruments such as IntelliCyt iQue Screener PLUS®. Here, we took advantage of this technology to transfer a previously validated cytotoxicity assay. The evaluated parameters were cell permeability, caspase activation and phosphatidyl serine exposure. The assay was accurate (r2 = 0.90), precise (%CV ≤ 18.90) and specific. These results showed that this technology is suitable to be used in control quality environments. In addition, the automation provided a faster acquisition and analysis of data with precise and accurate results. This application could be implemented to evaluate another in vitro mechanism of action of different biotherapeutics.

AB - Technological advances allowed the development of high-throughput instruments such as IntelliCyt iQue Screener PLUS®. Here, we took advantage of this technology to transfer a previously validated cytotoxicity assay. The evaluated parameters were cell permeability, caspase activation and phosphatidyl serine exposure. The assay was accurate (r2 = 0.90), precise (%CV ≤ 18.90) and specific. These results showed that this technology is suitable to be used in control quality environments. In addition, the automation provided a faster acquisition and analysis of data with precise and accurate results. This application could be implemented to evaluate another in vitro mechanism of action of different biotherapeutics.

KW - ADCC

KW - High-throughput flow cytometry

KW - Validation bioassay

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U2 - 10.1016/j.btre.2020.e00456

DO - 10.1016/j.btre.2020.e00456

M3 - Artículo

C2 - 32373484

AN - SCOPUS:85083851658

SN - 2215-017X

VL - 26

JO - Biotechnology Reports

JF - Biotechnology Reports

M1 - e00456

ER -

Taking advantage of a high-throughput flow cytometer for the implementation of an ADCC assay for regulatory compliance

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Keywords

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