TY - JOUR
T1 - Taenia solium
T2 - Immune response against oral or systemic immunization with purified recombinant calreticulin in mice
AU - Fonseca-Coronado, Salvador
AU - Ruiz-Tovar, Karina
AU - Pérez-Tapia, Mayra
AU - Mendlovic, Fela
AU - Flisser, Ana
N1 - Funding Information:
We are thankful to Mayra Cruz-Rivera, Sonia León-Cabrera, Guillermina Ávila-Ramirez, Yolanda Medina-Flores, Olga Mata-Ruiz, Benjamin Ortiz-López and Victor Rosales-García for technical assistance. The authors acknowledge DGAPA, UNAM for Grant PAPIIT IN220007. Salvador Foseca-Coronado was funded by Programa de Formación e Incorporación de Profesores de Carrera en Facultades y Escuelas para el Fortalecimiento de la Investigación (PROFIP). DGAPA, UNAM.
PY - 2011/1
Y1 - 2011/1
N2 - Recombinant functional Taenia solium calreticulin (rTsCRT) confers different degrees of protection in the experimental model of intestinal taeniosis in hamsters. The aim of this study was to evaluate the immune response induced after oral or systemic immunization with an electroeluted rTsCRT in BALB/c mice. Oral immunization elicited high fecal IgA and the production of IL-4 and IL-5 by mesenteric lymph node cells after in vitro stimulation with rTSCRT, indicating a Th2 response. Mice subcutaneously immunized produced high amounts of serum IgG, being IgG1 (Th2-related) the predominant isotype, while in vitro stimulated spleen cells synthesized IL-4, IL-5 and also IFN-γ, indicating a mixed Th1/Th2 cellular response after systemic immunization. Our data show that purified rTsCRT induces polarized Th2 responses after oral immunization of mice, a common characteristic of protective immunity against helminths and, consequently, a desirable hallmark in the search for a vaccine.
AB - Recombinant functional Taenia solium calreticulin (rTsCRT) confers different degrees of protection in the experimental model of intestinal taeniosis in hamsters. The aim of this study was to evaluate the immune response induced after oral or systemic immunization with an electroeluted rTsCRT in BALB/c mice. Oral immunization elicited high fecal IgA and the production of IL-4 and IL-5 by mesenteric lymph node cells after in vitro stimulation with rTSCRT, indicating a Th2 response. Mice subcutaneously immunized produced high amounts of serum IgG, being IgG1 (Th2-related) the predominant isotype, while in vitro stimulated spleen cells synthesized IL-4, IL-5 and also IFN-γ, indicating a mixed Th1/Th2 cellular response after systemic immunization. Our data show that purified rTsCRT induces polarized Th2 responses after oral immunization of mice, a common characteristic of protective immunity against helminths and, consequently, a desirable hallmark in the search for a vaccine.
KW - Calreticulin
KW - Cestode
KW - Mice
KW - Oral immunization
UR - http://www.scopus.com/inward/record.url?scp=78650531906&partnerID=8YFLogxK
U2 - 10.1016/j.exppara.2010.07.017
DO - 10.1016/j.exppara.2010.07.017
M3 - Artículo
SN - 0014-4894
VL - 127
SP - 313
EP - 317
JO - Experimental Parasitology
JF - Experimental Parasitology
IS - 1
ER -