Synthesis of hybrid materials, immobilization of lipase in SBA-15 modified with CaO

J. C. Méndez; U. Arellano; S. Solís; M. Asomoza; V. H. Lara; A. J. Padilla; J. A. Wang

doi:10.22201/icat.16656423.2018.16.6.747

Synthesis of hybrid materials, immobilization of lipase in SBA-15 modified with CaO

J. C. Méndez, U. Arellano, S. Solís, M. Asomoza, V. H. Lara, A. J. Padilla, J. A. Wang

Research output: Contribution to journal › Article › peer-review

2 Scopus citations

Abstract

Mesoporous silica materials (SBA-15) doped with calcium (Ca) at 1, 3 and 5% w/w were synthesized in order to immobilize Candida rugosa lipase (CRL), through the adsorption method. It was found that calcium (Ca) is present as CaO, highly dispersed on the surface of SBA-15, the specific area of SBA-15 decreases on incrementing CaO content. The environmental pH influences the adsorption of CRL due to the polarization of positive charges below the isoelectric point (IEP) whereas the Ca-SBA-15 materials present negative charges. The adsorption model proposed for CRL is about the accessible surface of the material, as this has a size of 7 nm and the Ca-SBA-15 present pores at an interval of 3 nm.

Original language	English
Pages (from-to)	498-510
Number of pages	13
Journal	Journal of Applied Research and Technology
Volume	16
Issue number	6
DOIs	https://doi.org/10.22201/icat.16656423.2018.16.6.747
State	Published - 2018
Externally published	Yes

Keywords

Biomaterials
CaO
Immobilization
Lipase
SBA-15

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10.22201/icat.16656423.2018.16.6.747

Cite this

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title = "Synthesis of hybrid materials, immobilization of lipase in SBA-15 modified with CaO",

abstract = "Mesoporous silica materials (SBA-15) doped with calcium (Ca) at 1, 3 and 5% w/w were synthesized in order to immobilize Candida rugosa lipase (CRL), through the adsorption method. It was found that calcium (Ca) is present as CaO, highly dispersed on the surface of SBA-15, the specific area of SBA-15 decreases on incrementing CaO content. The environmental pH influences the adsorption of CRL due to the polarization of positive charges below the isoelectric point (IEP) whereas the Ca-SBA-15 materials present negative charges. The adsorption model proposed for CRL is about the accessible surface of the material, as this has a size of 7 nm and the Ca-SBA-15 present pores at an interval of 3 nm.",

keywords = "Biomaterials, CaO, Immobilization, Lipase, SBA-15",

author = "M{\'e}ndez, {J. C.} and U. Arellano and S. Sol{\'i}s and M. Asomoza and Lara, {V. H.} and Padilla, {A. J.} and Wang, {J. A.}",

year = "2018",

doi = "10.22201/icat.16656423.2018.16.6.747",

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pages = "498--510",

journal = "Journal of Applied Research and Technology",

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TY - JOUR

T1 - Synthesis of hybrid materials, immobilization of lipase in SBA-15 modified with CaO

AU - Méndez, J. C.

AU - Arellano, U.

AU - Solís, S.

AU - Asomoza, M.

AU - Lara, V. H.

AU - Padilla, A. J.

AU - Wang, J. A.

PY - 2018

Y1 - 2018

N2 - Mesoporous silica materials (SBA-15) doped with calcium (Ca) at 1, 3 and 5% w/w were synthesized in order to immobilize Candida rugosa lipase (CRL), through the adsorption method. It was found that calcium (Ca) is present as CaO, highly dispersed on the surface of SBA-15, the specific area of SBA-15 decreases on incrementing CaO content. The environmental pH influences the adsorption of CRL due to the polarization of positive charges below the isoelectric point (IEP) whereas the Ca-SBA-15 materials present negative charges. The adsorption model proposed for CRL is about the accessible surface of the material, as this has a size of 7 nm and the Ca-SBA-15 present pores at an interval of 3 nm.

AB - Mesoporous silica materials (SBA-15) doped with calcium (Ca) at 1, 3 and 5% w/w were synthesized in order to immobilize Candida rugosa lipase (CRL), through the adsorption method. It was found that calcium (Ca) is present as CaO, highly dispersed on the surface of SBA-15, the specific area of SBA-15 decreases on incrementing CaO content. The environmental pH influences the adsorption of CRL due to the polarization of positive charges below the isoelectric point (IEP) whereas the Ca-SBA-15 materials present negative charges. The adsorption model proposed for CRL is about the accessible surface of the material, as this has a size of 7 nm and the Ca-SBA-15 present pores at an interval of 3 nm.

KW - Biomaterials

KW - CaO

KW - Immobilization

KW - Lipase

KW - SBA-15

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U2 - 10.22201/icat.16656423.2018.16.6.747

DO - 10.22201/icat.16656423.2018.16.6.747

M3 - Artículo

AN - SCOPUS:85074966225

SN - 1665-6423

VL - 16

SP - 498

EP - 510

JO - Journal of Applied Research and Technology

JF - Journal of Applied Research and Technology

IS - 6

ER -

Synthesis of hybrid materials, immobilization of lipase in SBA-15 modified with CaO

Abstract

Keywords

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