TY - JOUR
T1 - Synthesis and characterization of silver nanoparticles
T2 - Effect on phytopathogen Colletotrichum gloesporioides
AU - Aguilar-Méndez, Miguel A.
AU - Martín-Martínez, Eduardo San
AU - Ortega-Arroyo, Lesli
AU - Cobián-Portillo, Georgina
AU - Sánchez-Espíndola, Esther
N1 - Funding Information:
Acknowledgments This work was financially supported by Consejo Nacional de Ciencia y Tecnología (CONACYT) through project no. 90019 and SIP project no. 20082511. The authors would like to thank Dr. Geonel Gattorno for the technical assistance in electron diffraction and FTIR.
PY - 2011/6
Y1 - 2011/6
N2 - Colloidal silver nanoparticles were synthesized by reducing silver nitrate solutions with glucose, in the presenceof gelatin ascapping agent. The obtained nanoparticles were characterized by means of UV-Vis spectroscopy, transmission electron microscopy (TEM), and Fourier transform infrared (FTIR) spectroscopy. The response surface methodology (RSM) was also used to determine the influence of the variables on the sizeofthe nanoparticles. The antifungal activity of the silver nanoparticles was evaluated on the phytopathogen Colletotrichum gloesporioides, which causes anthracnose in a wide range of fruits. The UV-Vis spectra indicated the formation of silver nanoparticles preferably spherical and of relatively small size (<20 nm). The above-mentioned was confirmed by TEM, observing a size distribution of 5-24 nm. According to RSM the synthesis variables influenced onthe sizeofthe silver nanoparticles.Bymeans of FTIR spectroscopy it was determined that gelatin, through their amide and hydroxyl groups, interacts with nanoparticles preventing their agglomeration. The growth of C. gloesporioides in the presence of silver nanoparticles was significantly delayed in a dose dependent manner.
AB - Colloidal silver nanoparticles were synthesized by reducing silver nitrate solutions with glucose, in the presenceof gelatin ascapping agent. The obtained nanoparticles were characterized by means of UV-Vis spectroscopy, transmission electron microscopy (TEM), and Fourier transform infrared (FTIR) spectroscopy. The response surface methodology (RSM) was also used to determine the influence of the variables on the sizeofthe nanoparticles. The antifungal activity of the silver nanoparticles was evaluated on the phytopathogen Colletotrichum gloesporioides, which causes anthracnose in a wide range of fruits. The UV-Vis spectra indicated the formation of silver nanoparticles preferably spherical and of relatively small size (<20 nm). The above-mentioned was confirmed by TEM, observing a size distribution of 5-24 nm. According to RSM the synthesis variables influenced onthe sizeofthe silver nanoparticles.Bymeans of FTIR spectroscopy it was determined that gelatin, through their amide and hydroxyl groups, interacts with nanoparticles preventing their agglomeration. The growth of C. gloesporioides in the presence of silver nanoparticles was significantly delayed in a dose dependent manner.
KW - Antifungal activity
KW - Antimicrobial
KW - Colletotrichum gloesporiodes
KW - Gelatin
KW - Silver nanoparticles
UR - http://www.scopus.com/inward/record.url?scp=80052568661&partnerID=8YFLogxK
U2 - 10.1007/s11051-010-0145-6
DO - 10.1007/s11051-010-0145-6
M3 - Artículo
SN - 1388-0764
VL - 13
SP - 2525
EP - 2532
JO - Journal of Nanoparticle Research
JF - Journal of Nanoparticle Research
IS - 6
ER -