@article{6c3cdb27fde7489a96f56e032d58cb7c,
title = "Synthesis and application of a photoaffinity analog of dehydroepiandrosterone (DHEA)",
abstract = "We have synthesized an analog of dehydroepiandrosterone (DHEA, 1) containing both a benzophenone (BP) and a biotin (Bt) group (DHEA-BP-Bt, 8). Compound 8 was prepared by functionalization on C-17 of 1. Biocytin was reacted with 4-benzoylbenzoic acid and the product was condensed with 1 containing a diamine-hexane linker. We detected specific protein bands of approximately 55, 80, and 150 kDa by SDS-PAGE analysis of vascular endothelial cell plasma membranes which had been photoirradiated in the presence of 8.",
keywords = "Benzophenone, Biotin, Chemical probe, DHEA, Photoaffinity",
author = "Olivo, {Horacio F.} and Nury Perez-Hernandez and Dongmin Liu and Mary Iruthayanathan and Brianne O'Leary and Homan, {Laurie L.} and Dillon, {Joseph S.}",
note = "Funding Information: This material is based upon work supported by the Department of Veterans Affairs, Veterans Health Administration, Office of Research and Development, Biomedical Laboratory Research and Development Program. The contents do not represent the views of the Department of Veterans Affairs or the United States Government. The studies were also supported by Grants from the American Heart Association and the NIH ( AG018928 ). We thank Dr. Rebeca Lopez-Marure for critical reading of the manuscript. ",
year = "2010",
month = feb,
day = "1",
doi = "10.1016/j.bmcl.2009.12.019",
language = "Ingl{\'e}s",
volume = "20",
pages = "1153--1155",
journal = "Bioorganic and Medicinal Chemistry Letters",
issn = "0960-894X",
number = "3",
}