TY - JOUR
T1 - Synaptic vesicle protein 2 (SV2) isoforms
AU - Bandala, Cindy
AU - Miliar-García, Á
AU - Mejía-Barradas, C. M.
AU - Anaya-Ruiz, M.
AU - Luna-Arias, J. P.
AU - Bazán-Eḿndez, C. I.
AU - Gómez-López, M.
AU - Juárez-Méndez, S.
AU - Lara-Padilla, E.
PY - 2012
Y1 - 2012
N2 - New molecular markers of cancer had emerged with novel applications in cancer prevention and therapeutics, including for breast cancer of unknown causes, which has a high impact on the health of women worldwide. The purpose of this research was to detemine protein and mRNA expression of synaptic vesicle 2 (SV2) isoforms A, B and C in breast cancer cell lines. Cultured cell lines MDA-MB-231, SKBR3, T47D were lysed and their protein and mRNA expression analyzed by real-time PCR and western blot technique, respectively. SV2A, B proteins were identified in non-tumor (MCF-10A) and tumor cell lines (MDA-MB-231 and T47D) while SV2C only was found in the T47D cell line. Furthermore, the genomic expression was consistent with protein expression for a such cell line, but in MDA-MB-231 there was no SV2B genomic expression, and the SV2C mRNA and protein were not found in the non tumoral cell line. These findings suggest a possible cellular transdifferentiation to neural character in breast cancer, of possible relevance to cancer development, and point to possible use of SV2 as molecular marker and a vehicle for cancer treatment with botulinum toxin.
AB - New molecular markers of cancer had emerged with novel applications in cancer prevention and therapeutics, including for breast cancer of unknown causes, which has a high impact on the health of women worldwide. The purpose of this research was to detemine protein and mRNA expression of synaptic vesicle 2 (SV2) isoforms A, B and C in breast cancer cell lines. Cultured cell lines MDA-MB-231, SKBR3, T47D were lysed and their protein and mRNA expression analyzed by real-time PCR and western blot technique, respectively. SV2A, B proteins were identified in non-tumor (MCF-10A) and tumor cell lines (MDA-MB-231 and T47D) while SV2C only was found in the T47D cell line. Furthermore, the genomic expression was consistent with protein expression for a such cell line, but in MDA-MB-231 there was no SV2B genomic expression, and the SV2C mRNA and protein were not found in the non tumoral cell line. These findings suggest a possible cellular transdifferentiation to neural character in breast cancer, of possible relevance to cancer development, and point to possible use of SV2 as molecular marker and a vehicle for cancer treatment with botulinum toxin.
KW - BoNTA
KW - Breast
KW - Cancer
KW - Protein/gene expression
KW - SV2A
KW - SV2B
KW - SV2C
UR - http://www.scopus.com/inward/record.url?scp=84874008931&partnerID=8YFLogxK
U2 - 10.7314/APJCP.2012.13.10.5063
DO - 10.7314/APJCP.2012.13.10.5063
M3 - Artículo
SN - 1513-7368
VL - 13
SP - 5063
EP - 5067
JO - Asian Pacific Journal of Cancer Prevention
JF - Asian Pacific Journal of Cancer Prevention
IS - 10
ER -