TY - JOUR
T1 - Survival and malformations rates, oxidative status in early life stages of Cyprinus carpio due to exposure to environmentally realistic concentrations of paracetamol
AU - Gutiérrez-Noya, Verónica Margarita
AU - Gómez-Oliván, Leobardo Manuel
AU - Ramírez-Montero, María del Carmen
AU - Islas-Flores, Hariz
AU - Galar-Martínez, Marcela
AU - García-Medina, Sandra
N1 - Publisher Copyright:
© 2021 Elsevier B.V.
PY - 2021/5/10
Y1 - 2021/5/10
N2 - Paracetamol (PCM) is among the most consumed analgesic and antipyretic drugs worldwide. Due to its high consumption, this drug has been reported ubiquitously on different water bodies, posing a real threat to aquatic organisms. Until now, several studies have pointed out that PCM may induce oxidative stress, histological damage and developmental disorders on different aquatic species. Nonetheless, there is still a huge knowledge gap about the toxic effects that PCM may induce in species of commercial interest such as the common carp Cyprinus carpio. The aim of this study was to evaluate survival and malformation rates induced by PCM (0.5 μg/L – 3.5 μg/L) in early life stages of common carp. Furthermore, oxidative stress biomarkers were evaluated at 72 and 96 h post fecundation. PCM reduced the survival rate of the embryos of up to 90%, as concentration increased. LC50 and EC50m were 1.29 μg/L and 2.84 μg/L, respectively. Biomarkers of cellular oxidation and antioxidant enzymes were modified in a concentration-dependent way with respect to the control group (p < 0.05). The main developmental alterations observed were lordosis, scoliosis, craniofacial malformations, hypopigmentation, growth retardation, pericardial edema and rachyschisis. These data indicate that environmentally realistic concentrations of PCM could be hazardous and affects the development in early stages of C. carpio. Moreover, our findings also indicate that C. carpio embryos may be a useful in vivo model to evaluate embryonic and teratogenic effects of drugs such as PCM.
AB - Paracetamol (PCM) is among the most consumed analgesic and antipyretic drugs worldwide. Due to its high consumption, this drug has been reported ubiquitously on different water bodies, posing a real threat to aquatic organisms. Until now, several studies have pointed out that PCM may induce oxidative stress, histological damage and developmental disorders on different aquatic species. Nonetheless, there is still a huge knowledge gap about the toxic effects that PCM may induce in species of commercial interest such as the common carp Cyprinus carpio. The aim of this study was to evaluate survival and malformation rates induced by PCM (0.5 μg/L – 3.5 μg/L) in early life stages of common carp. Furthermore, oxidative stress biomarkers were evaluated at 72 and 96 h post fecundation. PCM reduced the survival rate of the embryos of up to 90%, as concentration increased. LC50 and EC50m were 1.29 μg/L and 2.84 μg/L, respectively. Biomarkers of cellular oxidation and antioxidant enzymes were modified in a concentration-dependent way with respect to the control group (p < 0.05). The main developmental alterations observed were lordosis, scoliosis, craniofacial malformations, hypopigmentation, growth retardation, pericardial edema and rachyschisis. These data indicate that environmentally realistic concentrations of PCM could be hazardous and affects the development in early stages of C. carpio. Moreover, our findings also indicate that C. carpio embryos may be a useful in vivo model to evaluate embryonic and teratogenic effects of drugs such as PCM.
KW - Common carp
KW - Developmental disturbances
KW - Oxidative status
KW - Paracetamol
KW - Teratogenesis
UR - http://www.scopus.com/inward/record.url?scp=85100134720&partnerID=8YFLogxK
U2 - 10.1016/j.scitotenv.2020.144585
DO - 10.1016/j.scitotenv.2020.144585
M3 - Artículo
C2 - 33454465
AN - SCOPUS:85100134720
SN - 0048-9697
VL - 768
JO - Science of the Total Environment
JF - Science of the Total Environment
M1 - 144585
ER -