TY - JOUR
T1 - Sulfate reduction and trichloroethylene biodegradation by a marine microbial community from hydrothermal vents sediments
AU - Guerrero-Barajas, Claudia
AU - Garibay-Orijel, Claudio
AU - Rosas-Rocha, Liliana E.
N1 - Funding Information:
The authors are grateful for the financial support provided by the National Council of Science and Technology in Mexico (CONACYT grant number 82627 ) and master’s scholarship awarded to Liliana-E. Rosas-Rocha (CONACYT- 242656), as well as to Distrito Federal’s Institute of Science and Technology (ICYT, DF grant PICS08-79 ). Finally, thanks also to the Centro Regional de Investigación Pesquera (CRIP) Bahía de Banderas, Nayarit, Mexico for the technical assistance provided during the collection of the sediment samples.
PY - 2011/1
Y1 - 2011/1
N2 - Sulfate reduction (SR) and trichloroethylene (TCE) biodegradation at two different temperatures (37 and 70°C) were investigated in enrichment cultures prepared with two different samples of sediments collected from hydrothermal vents. The unadapted sediments were incubated with sulfate (4gL-1) as the electron acceptor before TCE addition to enrich them in biomass and to establish a constant sulfate reduction (SR, 87% sulfate conversion and specific H2S concentration of 90.81±8.19mgH2SgVSS-1), afterwards TCE was added at an initial concentration of 300μmolL-1. The best results for TCE biodegradation were obtained at 37°C. At this temperature, SR was up to 92%, whereas TCE biodegradation reached 75% and ethane was detected as the main degradation product. Under thermophilic conditions (70°C) TCE biodegradation reached up to approximately 60% and the SR was 30% in 30 days of incubation with the chlorinated solvent. Along with these results, the 16S rDNA analysis from samples at 37°C showed the presence of bacteria belonging to the genera: Clostridium, Bacillus and Desulfuromonas. The overall results on TCE degradation and SR suggest that cometabolic TCE degradation is carried out by sulfate or sulfur reducers and fermentative bacteria at mesophilic conditions.
AB - Sulfate reduction (SR) and trichloroethylene (TCE) biodegradation at two different temperatures (37 and 70°C) were investigated in enrichment cultures prepared with two different samples of sediments collected from hydrothermal vents. The unadapted sediments were incubated with sulfate (4gL-1) as the electron acceptor before TCE addition to enrich them in biomass and to establish a constant sulfate reduction (SR, 87% sulfate conversion and specific H2S concentration of 90.81±8.19mgH2SgVSS-1), afterwards TCE was added at an initial concentration of 300μmolL-1. The best results for TCE biodegradation were obtained at 37°C. At this temperature, SR was up to 92%, whereas TCE biodegradation reached 75% and ethane was detected as the main degradation product. Under thermophilic conditions (70°C) TCE biodegradation reached up to approximately 60% and the SR was 30% in 30 days of incubation with the chlorinated solvent. Along with these results, the 16S rDNA analysis from samples at 37°C showed the presence of bacteria belonging to the genera: Clostridium, Bacillus and Desulfuromonas. The overall results on TCE degradation and SR suggest that cometabolic TCE degradation is carried out by sulfate or sulfur reducers and fermentative bacteria at mesophilic conditions.
KW - Biodegradation
KW - Hydrothermal vents
KW - Sulfate reduction
KW - Thermophilic
KW - Trichloroethylene
UR - http://www.scopus.com/inward/record.url?scp=78650677254&partnerID=8YFLogxK
U2 - 10.1016/j.ibiod.2010.10.004
DO - 10.1016/j.ibiod.2010.10.004
M3 - Artículo
SN - 0964-8305
VL - 65
SP - 116
EP - 123
JO - International Biodeterioration and Biodegradation
JF - International Biodeterioration and Biodegradation
IS - 1
ER -