TY - JOUR
T1 - Subcellular distribution of the Entamoeba histolytica 140 kDa FN-binding molecule during host-parasite interaction
AU - Hernández-Ramírez, V. I.
AU - Rios, A.
AU - Angel, A.
AU - Magos, M. A.
AU - Pérez-Castillo, L.
AU - Rosales-Encina, J. L.
AU - Castillo-Henkel, E.
AU - Talamás-Rohana, P.
PY - 2007/2
Y1 - 2007/2
N2 - Entamoeba histolytica trophozoites recovered from the host-parasite interface during abscess development obtain different stimuli compared with long-term cultured cells. In order to have a better understanding about the mechanisms in which the 140 kDa fibronectin (FN)-binding molecule (EhFNR) is involved during the invasive process, we decided to compare the regulation process of this molecule among long-term cultured trophozoites, FN-stimulated trophozoites, and trophozoites recently recovered from a liver abscess. A cDNA clone (5A) containing a fragment of the EhFNR that shows identity to the C-terminal region of the intermediate galactose lectin subunit Igl, was selected with a mAb (3C10). Identity of EhFNR with Igl was confirmed by immunoprecipitation with 3C10 and EH3015 (against the Gal/GalNAc intermediate subunit) mAbs. The 3C10 mAb was used as a tool to explore the modulation of the amoebic receptor (EhFNR). Our results showed specific regulation of the EhFNR in FN-interacted amoebas, as well as in trophozoites recovered at different stages of abscess development. This regulation involved mobilization of the receptor molecule from internal vesicles to the plasma membrane. Therefore, we suggest that in the host-parasite interface, the EhFNR (Igl) plays an important role in the adhesion process during abscess development.
AB - Entamoeba histolytica trophozoites recovered from the host-parasite interface during abscess development obtain different stimuli compared with long-term cultured cells. In order to have a better understanding about the mechanisms in which the 140 kDa fibronectin (FN)-binding molecule (EhFNR) is involved during the invasive process, we decided to compare the regulation process of this molecule among long-term cultured trophozoites, FN-stimulated trophozoites, and trophozoites recently recovered from a liver abscess. A cDNA clone (5A) containing a fragment of the EhFNR that shows identity to the C-terminal region of the intermediate galactose lectin subunit Igl, was selected with a mAb (3C10). Identity of EhFNR with Igl was confirmed by immunoprecipitation with 3C10 and EH3015 (against the Gal/GalNAc intermediate subunit) mAbs. The 3C10 mAb was used as a tool to explore the modulation of the amoebic receptor (EhFNR). Our results showed specific regulation of the EhFNR in FN-interacted amoebas, as well as in trophozoites recovered at different stages of abscess development. This regulation involved mobilization of the receptor molecule from internal vesicles to the plasma membrane. Therefore, we suggest that in the host-parasite interface, the EhFNR (Igl) plays an important role in the adhesion process during abscess development.
KW - Entamoeba histolytica
KW - Fibronectin
KW - Fibronectin-binding molecule
KW - Gal/GalNAc intermediate subunit
KW - Host-parasite interface
KW - Vesicle mobilization
UR - http://www.scopus.com/inward/record.url?scp=33846006246&partnerID=8YFLogxK
U2 - 10.1017/S0031182006001260
DO - 10.1017/S0031182006001260
M3 - Artículo
C2 - 17076927
SN - 0031-1820
VL - 134
SP - 169
EP - 177
JO - Parasitology
JF - Parasitology
IS - 2
ER -